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Title

Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit

Authors
Barkas, T.
Gabriel, J.M.
Hughes, G.J.
Roth, B.
Alliod, C.
Tzartos, S.J.
Ballivet, M.
Published in Journal of Biological Chemistry. 1988, vol. 263, no. 12, p. 5916-5920
Abstract Monoclonal antibodies (mAbs) to the main immunogenic region (MIR) bind to fusion proteins containing region 37-200 of the alpha chain of Torpedo, mouse, and chicken nicotinic acetylcholine receptor. In the case of the mouse alpha chain, these mAbs react with sequence 61-216 but not with 74-216. A synthetic peptide M1, containing residues 61-76 of the mouse alpha chain, also binds these anti-MIR mAbs, showing that all or part of their binding site is included in this region. The conformational dependence and epitope specificity of the mAbs are discussed.
Keywords Amino acid sequenceAnimalsAntibodies, monoclonal immunologyBinding sites antibodyChickensElectrophoresis polyacrylamide gelEpitopes immunologyImmunoassayMiceMolecular sequence dataPeptide fragments immunologyRatsReceptors nicotinic immunologyRecombinant fusion proteins immunologyTorpedo
Identifiers
PMID: 2451673
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(ISO format)
BARKAS, T. et al. Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit. In: Journal of Biological Chemistry, 1988, vol. 263, n° 12, p. 5916-5920. https://archive-ouverte.unige.ch/unige:85387

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