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Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit

Published inThe Journal of biological chemistry, vol. 263, no. 12, p. 5916-5920
Publication date1988
Abstract

Monoclonal antibodies (mAbs) to the main immunogenic region (MIR) bind to fusion proteins containing region 37-200 of the alpha chain of Torpedo, mouse, and chicken nicotinic acetylcholine receptor. In the case of the mouse alpha chain, these mAbs react with sequence 61-216 but not with 74-216. A synthetic peptide M1, containing residues 61-76 of the mouse alpha chain, also binds these anti-MIR mAbs, showing that all or part of their binding site is included in this region. The conformational dependence and epitope specificity of the mAbs are discussed.

Keywords
  • Amino acid sequence
  • Animals
  • Antibodies, monoclonal immunology
  • Binding sites antibody
  • Chickens
  • Electrophoresis polyacrylamide gel
  • Epitopes immunology
  • Immunoassay
  • Mice
  • Molecular sequence data
  • Peptide fragments immunology
  • Rats
  • Receptors nicotinic immunology
  • Recombinant fusion proteins immunology
  • Torpedo
Affiliation Not a UNIGE publication
Citation (ISO format)
BARKAS, T. et al. Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit. In: The Journal of biological chemistry, 1988, vol. 263, n° 12, p. 5916–5920.
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Article (Published version)
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Identifiers
ISSN of the journal0021-9258
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