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Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression

Published inMolecular and cellular biology, vol. 1, no. 7, p. 661-671
Publication date1981
Abstract

We have examined histone gene expression during the early stages of sea urchin embryogenesis. The five histone genes expressed at that time are contained in tandem repetitive segments. It has been suggested that adjacent coding regions and their intervening spacer sequences are transcribed into large polycistronic messenger ribonucleic acid (RNA) precursors. We have subcloned into pBR322 deoxyribonucleic acid (DNA) sequences mapping either in the coding region, the 5' spacer, or the 3' spacer of the H2B histone gene. These clones were used to produce radioiodinated hybridization probes. We measured the steady-state quantity of H2B messenger RNA as well as spacer-specific RNA in the total RNA from embryos taken at various stages of development from fertilization to hatching of blastulae (0 to 22 h post-fertilization). Small amounts of RNA hybridizing to both spacer probes could be found. However, we show that these RNAs form mismatched hybrids with the spacer DNA and therefore cannot originate from the spacers present in the histone genes. We conclude that there is no detectable transcription of the spacer regions on either side of the H2B histone gene. The detection limit for RNA complementary to the 5' spacer sequence corresponds to a maximum of about three RNA molecules per cell, an amount shown to be far less than the projected steady-state pool size of a putative polycistronic transcript, if such a precursor were to be the obligatory transcript of the histone genes. (This conclusion was derived by using the known rates of production of H2B mRNA throughout early development [R. E. Maxson and F. H. Wilt, Dev. Biol., in press].) The physiologically relevant transcript of the histone genes in early development is therefore monocistronic and probably identical to the messenger RNA itself.

Keywords
  • Animals
  • Base sequence
  • Blotting northern
  • DNA ribosomal metabolism
  • Gene expression
  • Histones genetics
  • Molecular sequence data
  • Nucleic acid hybridization
  • RNA messenger metabolism
  • Restriction mapping
  • Sea urchins embryology genetics
  • Transcription genetic
NotePMCID: PMC369713
Affiliation Not a UNIGE publication
Citation (ISO format)
MAURON, Alex et al. Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression. In: Molecular and cellular biology, 1981, vol. 1, n° 7, p. 661–671.
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Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0270-7306
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