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Title

Effects of a mechanical stimulation on localization of annexin-like proteins in Bryonia dioica internodes

Authors
Thonat, C.
Mathieu, C.
Boyer, N.
Published in Plant Physiology. 1997, vol. 114, no. 3, p. 981-988
Abstract Mechanical stimulation exerted by rubbing a young internode of Bryonia dioica plants inhibits its growth. Previous cellular and biochemical studies showed that this growth inhibition is associated with Ca2+ redistribution and profound modifications of plasma membrane characteristics. We extracted and purified Ca2+-dependent phospholipid-binding proteins from B. dioica internodes. Two main proteins, p33 and p35, and other minor bands were isolated and identified as annexin-like proteins because of their biochemical properties and their cross-reactions with antibodies against maize (Zea mays L.) annexins. Rabbit antiserum was obtained by injection of B. dioica p35. This antiserum was used for the immunocytolocalization of annexin-like proteins in internode parenchyma cells. It appeared that the distribution of annexin-like proteins was different before and 30 min after the mechanical stimulation. Western analysis of proteins in membrane fractions after separation by free-flow electrophoresis showed that p35 was present in most fractions, whereas p33 appeared mainly in plasmalemma-enriched fractions after the mechanical stimulation. It is hypothesized that a subcellular redistribution of these proteins might be involved in growth inhibition by mechanical stress.
Keywords Phospholipid-binding proteinsFree-flow electrophoresis, plasma-membrane vesiclesEthylene productionSoybean hypocotylsSequence-analysisPlant annexinsEndonexin-IICalciumThigmomorphogenesis
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THONAT, C. et al. Effects of a mechanical stimulation on localization of annexin-like proteins in Bryonia dioica internodes. In: Plant Physiology, 1997, vol. 114, n° 3, p. 981-988. https://archive-ouverte.unige.ch/unige:8529

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Deposited on : 2010-07-02

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