Professional Article
previous document  unige:85109  next document
add to browser collection
Title

Kinetics of synthesis of cytoplasmic messenger-like RNA not associated with ribosomes in HeLa cells

Authors
Published in European Journal of Biochemistry. 1978, vol. 82, no. 2, p. 619-625
Abstract The turn-over of cytoplasmic messenger-like RNA not associated with polyribosomes as well as that of polyribosomal mRNA was investigated by labelling with [3H]uridine in conditions of arrested ribosomal RNA and mitochondrial RNA synthesis. The synthesis of ribosomal RNA was inhibited with toyokamycin and that of mitochondrial RNA with ethidium bromide. In both accumulation kinetics and actinomycin-D-chase experiments, cytoplasmic messenger-like ribonucleoprotein particles and polyribosomes were fractionated by buoyant density centrifugation in CsCl gradients. The half-life of free m1RNA was found to be of 1--2 h whereas the bulk of polyribosomal mRNA was stable over the time period considered (up to 8 h) but with a minor short-lived component. Purification of RNA from polyribosomes labelled under the same conditions and fractionation of it into polyadenylated and non-polyadenylated fractions showed that this short-lived minor component of half-life less than 1 h is non-polyadenylated.
Keywords Cytoplasm metabolismHela cells metabolismKineticsMathematicsMolecular weightPoly a metabolismRNA messenger biosynthesisRNA neoplasm biosynthesisRibosomes metabolism
Identifiers
PMID: 624291
Full text
Citation
(ISO format)
MAURON, Alex, SPOHR, Georges. Kinetics of synthesis of cytoplasmic messenger-like RNA not associated with ribosomes in HeLa cells. In: European Journal of Biochemistry, 1978, vol. 82, n° 2, p. 619-625. https://archive-ouverte.unige.ch/unige:85109

74 hits

29 downloads

Update

Deposited on : 2016-07-11

Export document
Format :
Citation style :