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Title

Cytosolic-free calcium in smooth-muscle and endothelial cells in an intact arterial wall from rat mesenteric artery in vitro

Authors
Schuster, A.
Stergiopulos, N.
Meister, J.J.
Published in Cell Calcium. 2001, vol. 30, no. 4, p. 261-267
Abstract The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examined in an intact arterial wall in vitro. Strips of the main branch of rat mesenteric artery were used. Cytosolic-free calcium concentration [Ca2+]i was estimated by determining the fluorescence ratio of the two calcium probes, Fluo-4 and Fura red. The emitted fluorescence of both probes was measured with a confocal microscope. We showed that potassium and phenylephrine, which increase the cytosolic -free calcium concentration of the smooth-muscle cells, also indirectly influence the calcium concentration in the endothelial cells. By simultaneously determining [Ca2+]i in the endothelial and the smooth-muscle cells of an arterial strip, we observed that when calcium increases in the endothelial cells in response to acetylcholine, it slightly decreases in the smooth-muscle cells. We conclude that the regulation of [Ca2+]i in the arterial endothelial cell, depends according to the stimuli either upon the endothelial cells themselves, or upon the coupled smooth-muscle cells.
Keywords Acetylcholine/pharmacologyAdrenergic alpha-Agonists/pharmacologyAniline Compounds/analysisAnimalsBenzofurans/analysisCalcium/metabolismCytosol/metabolismEndotheliumVascular/drug effects/metabolismFluorescent Dyes/analysisImidazoles/analysisIn Vitro TechniquesMaleMesenteric ArteriesMuscleSmooth/drug effects/metabolismPhenylephrine/pharmacologyPotassium/pharmacologyRatsRatsSprague-DawleyVasodilator Agents/pharmacologyXanthenes/analysis
Identifiers
PMID: 11587550
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OISHI, Hirotaka et al. Cytosolic-free calcium in smooth-muscle and endothelial cells in an intact arterial wall from rat mesenteric artery in vitro. In: Cell Calcium, 2001, vol. 30, n° 4, p. 261-267. https://archive-ouverte.unige.ch/unige:85060

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Deposited on : 2016-07-06

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