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Title

Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy

Authors
Wataha, John C
Lockwood, Petra E
Galgano, Camillo
Golay, A
Published in European Journal of Oral Sciences. 2004, vol. 112, no. 2, p. 182-7
Abstract The objectives of this study were to evaluate the cytotoxicity and sealing properties of four classes of endodontic sealers (PCS/Kerr, RoekoSeal/Roeko, TopSeal/Dentsply, and EndoREZ/Ultradent). For cytotoxicity testing (MTT method), the materials were either placed immediately in contact with cultured cells or 24 h after setting, then evaluated at three subsequent time points (24 h, 48 h, or 1 wk). For the leakage study, extracted human roots were obturated with acrylic cones and sealers and immersed for 48 h into rhodamine-labeled lipopolysaccharide. The roots were then observed under a confocal laser scanning microscope to estimate (semiquantitatively) the presence of the rhodamine-lipopolysaccharide (LPS) inside the canal. The results showed that cytotoxicity generally increased with time, and that most materials pose significant cytotoxic risks, particularly in the freshly mixed condition. Further, all materials showed significant leakage although there was large variation among teeth. Overall, the silicon-based material (Roeko Seal) was less cytotoxic and more effective in sealing root canals against LPS leakage than other materials.
Keywords Analysis of VarianceAnimalsBALB 3T3 CellsCarbamates/toxicityColoring AgentsComposite Resins/toxicityDental BondingDental Cements/toxicityDental Leakage/classificationEpoxy Resins/toxicityFluorescent DyesHumansLipopolysaccharides/pharmacokineticsMaterials TestingMiceMicroscopyConfocalPolyesters/toxicityRhodaminesRoot Canal Filling Materials/classification/toxicityRoot Canal ObturationSuccinate Dehydrogenase/drug effectsSurface PropertiesTetrazolium SaltsThiazolesTime FactorsCytotoxicitySealingEndodontic sealersConfocal Microscopy
Identifiers
PMID: 15056117
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Article (Published version) (516 Kb) - public document Free access
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Research group Groupe Krejci Ivo (médecine dentaire) (240)
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BOUILLAGUET, Serge et al. Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy. In: European Journal of Oral Sciences, 2004, vol. 112, n° 2, p. 182-7. https://archive-ouverte.unige.ch/unige:85015

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Deposited on : 2016-07-04

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