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Title

Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy
Authors
Bouillaguet, Serge
Wataha, John C
Lockwood, Petra E
Galgano, Camillo
Golay, A
Krejci, Ivo
Published in European journal of oral sciences. 2004, vol. 112, no. 2, p. 182-7
Abstract The objectives of this study were to evaluate the cytotoxicity and sealing properties of four classes of endodontic sealers (PCS/Kerr, RoekoSeal/Roeko, TopSeal/Dentsply, and EndoREZ/Ultradent). For cytotoxicity testing (MTT method), the materials were either placed immediately in contact with cultured cells or 24 h after setting, then evaluated at three subsequent time points (24 h, 48 h, or 1 wk). For the leakage study, extracted human roots were obturated with acrylic cones and sealers and immersed for 48 h into rhodamine-labeled lipopolysaccharide. The roots were then observed under a confocal laser scanning microscope to estimate (semiquantitatively) the presence of the rhodamine-lipopolysaccharide (LPS) inside the canal. The results showed that cytotoxicity generally increased with time, and that most materials pose significant cytotoxic risks, particularly in the freshly mixed condition. Further, all materials showed significant leakage although there was large variation among teeth. Overall, the silicon-based material (Roeko Seal) was less cytotoxic and more effective in sealing root canals against LPS leakage than other materials.
Keywords Analysis of Variance — Animals — BALB 3T3 Cells — Carbamates/toxicity — Coloring Agents — Composite Resins/toxicity — Dental Bonding — Dental Cements/toxicity — Dental Leakage/classification — Epoxy Resins/toxicity — Fluorescent Dyes — Humans — Lipopolysaccharides/pharmacokinetics — Materials Testing — Mice — Microscopy — Confocal — Polyesters/toxicity — Rhodamines — Root Canal Filling Materials/classification/toxicity — Root Canal Obturation — Succinate Dehydrogenase/drug effects — Surface Properties — Tetrazolium Salts — Thiazoles — Time Factors — Cytotoxicity — Sealing — Endodontic sealers — Confocal Microscopy
Identifiers
PMID: 15056117
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Article (Published version) (516 Kb) - public document Free access
Structures
Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de cariologie et d'endodontie
Research group Groupe Krejci Ivo (médecine dentaire) (240)
Citation
(ISO format) 		BOUILLAGUET, Serge et al. Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy. In: European journal of oral sciences, 2004, vol. 112, n° 2, p. 182-7. doi: 10.1111/j.1600-0722.2004.00115.x https://archive-ouverte.unige.ch/unige:85015

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Deposited on : 2016-07-04

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