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Scientific article
Open access
English

Surveying the Delivery Methods of CRISPR/Cas9 for ex vivo Mammalian Cell Engineering

Published inChimia, vol. 70, no. 6, p. 439-442
Publication date2016
Abstract

The simplicity of the CRISPR/Cas9 technology has been transformative in making targeted genome editing accessible for laboratories around the world. However, due to the sheer volume of literature generated in the past five years, determining the best format and delivery method of CRISPR/Cas9 components can be challenging. Here, we provide a brief overview of the progress that has been made in the ex vivo genome editing of mammalian cells and summarize the key advances made for improving efficiency and delivery of CRISPR/Cas9 in DNA, RNA, and protein form. In particular, we highlight the delivery of Cas9 components to human cells for advanced genome editing applications such as large gene insertion.

Keywords
  • Cas9
  • CRISPR
  • Genome editing
Research group
Citation (ISO format)
KELTON, William J. et al. Surveying the Delivery Methods of CRISPR/Cas9 for ex vivo Mammalian Cell Engineering. In: Chimia, 2016, vol. 70, n° 6, p. 439–442. doi: 10.2533/chimia.2016.439
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Article (Published version)
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Article (Published version)
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Identifiers
ISSN of the journal0009-4293
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Technical informations

Creation06/29/2016 3:15:00 PM
First validation06/29/2016 3:15:00 PM
Update time03/15/2023 12:31:17 AM
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