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Strategic approaches for overcoming peptide and protein instability within biodegradable nano- and microparticles

Publié dansEuropean journal of pharmaceutics and biopharmaceutics, vol. 59, no. 3, p. 375-388
Date de publication2005
Résumé

This paper reviews the major factors that are closely involved in peptide and protein degradation during the preparation of biodegradable nano- and microparticles. The various means usually employed for overcoming these obstacles are described, in order to bring to the fore the strategies for protein stabilization. Both processing and formulation parameters can be modified and are distinctly considered from a strategic point of view. We describe how partial or full protein stability retention within the carriers and during drug release might be achieved by individual or combined optimized strategies. Additionally, problems commonly encountered during protein quantification, stability determination and release are briefly reviewed. Artefacts that might occur during sampling and analytical procedures and which might hinder critical interpretation of results are discussed.

Mots-clés
  • Biodegradable polymers
  • Nanoparticles
  • Microparticles
  • Peptides
  • PLGA
  • PLA
  • Proteins
  • Stabilization
Groupe de recherche
Citation (format ISO)
BILATI, Ugo, ALLÉMANN, Eric, DOELKER, Eric. Strategic approaches for overcoming peptide and protein instability within biodegradable nano- and microparticles. In: European journal of pharmaceutics and biopharmaceutics, 2005, vol. 59, n° 3, p. 375–388. doi: 10.1016/j.ejpb.2004.10.006
Fichiers principaux (1)
Article (Published version)
accessLevelRestricted
Identifiants
ISSN du journal0939-6411
426vues
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Informations techniques

Création07/06/2016 13:40:00
Première validation07/06/2016 13:40:00
Heure de mise à jour15/03/2023 00:30:01
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