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Title

Disruption of Kcc2-dependent inhibition of olfactory bulb output neurons suggests its importance in odour discrimination

Authors
Gödde, Kathrin
Puchkov, Dmytro
Pfeffer, Carsten
Jentsch, Thomas
Published in Nature Communications. 2016, vol. 7, no. 12043, p. 1-13
Abstract Synaptic inhibition in the olfactory bulb (OB), the first relay station of olfactory information, is believed to be important for odour discrimination. We interfered with GABAergic inhibition of mitral and tufted cells (M/T cells), the principal neurons of the OB, by disrupting their potassium-chloride cotransporter 2 (Kcc2). Roughly, 70% of mice died around 3 weeks, but surviving mice appeared normal. In these mice, the resulting increase in the intracellular Cl_ concentration nearly abolished GABA-induced hyperpolarization of mitral cells (MCs) and unexpectedly increased the number of perisomatic synapses on MCs. In vivo analysis of odorant-induced OB electrical activity revealed increased M/Tcell firing rate, altered phasing of action potentials in the breath cycle and disrupted separation of odour-induced M/T cell activity patterns. Mice also demonstrated a severely impaired ability to discriminate chemically similar odorants or odorant mixtures. Our work suggests that precisely tuned GABAergic inhibition onto M/Tcells is crucial for M/Tcell spike pattern separation needed to distinguish closely similar odours.
Keywords Olfactory bulbPattern separationChloride gradient
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Research group Groupe Carleton Alan (neurosciences fondamentales) (876)
Project FNS: 31003A_153410
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GÖDDE, Kathrin et al. Disruption of Kcc2-dependent inhibition of olfactory bulb output neurons suggests its importance in odour discrimination. In: Nature Communications, 2016, vol. 7, n° 12043, p. 1-13. https://archive-ouverte.unige.ch/unige:84786

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Deposited on : 2016-06-28

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