UNIGE document Scientific Article
previous document  unige:84279  next document
add to browser collection
Title

Nicotinic acetylcholine receptor gene expression in developing chick autonomic ganglia

Authors
Published in European Journal of Pharmacology. 2000, vol. 393, no. 1-3, p. 97-104
Abstract The developmental expression patterns of ten genes encoding nicotinic acetylcholine receptor subunits were analyzed using Northern blots and in situ hybridization in chick peripheral ganglia of neural crest, placodal and dual embryonic origin. The superior cervical and ciliary ganglia were investigated in detail because they accumulated relatively abundant transcripts of the alpha3, beta4, alpha5 and alpha7 genes. In the superior cervical ganglion, these four mRNA species had similar developmental time-courses. They appeared at embryonic day 8 (E8), increased steadily until E16 and maintained a rather high plateau level until E18. In the ciliary ganglion, alpha7 transcripts were already abundant at E6, increased until E10, and considerably decreased thereafter. High-resolution in situ hybridization showed that alpha7 transcripts were present in all cell types of the E6 ciliary ganglion, whereas they were restricted to large neuronal somas at E16. Transfections with a reporter gene under the control of the alpha7 promoter demonstrated that a sharp developmental divide occurred at E11-12, after which stage the promoter was activatable in neurons exclusively.
Keywords Acetylcholine receptorLigand-gated ion channelRNA blotIn situ hybridizationPeripheral nervous systemNeural development
Identifiers
PMID: 10771002
Full text
Article (Published version) (1.7 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
ERKMAN, Linda et al. Nicotinic acetylcholine receptor gene expression in developing chick autonomic ganglia. In: European Journal of Pharmacology, 2000, vol. 393, n° 1-3, p. 97-104. https://archive-ouverte.unige.ch/unige:84279

84 hits

0 download

Update

Deposited on : 2016-06-07

Export document
Format :
Citation style :