Scientific article

Phosphorylation of GDI and membrane cycling of rab proteins

Published inFEBS letters, vol. 329, no. 3, p. 313-318
Publication date1993

Membrane transport is known to be regulated by protein phosphorylation and by small GTPases of the rab family. Using specific antibodies, we have identified a 55 kDa phosphorylated protein which co-immunoprecipitated with the cytosolic forms of rab5 and other rab proteins. We demonstrate, on the basis of its mobility in two-dimensional electrophoresis gels and its immunological properties, that this protein is rab GDI (p55/GDI). We also found that, a minor fraction of p55/GDI is membrane associated, but, whilst also complexed with rab proteins, it is not phosphorylated. On the basis of these data we suggest that the cycling of rab proteins between membranes and cytosol is regulated by phosphorylation of p55/GDI.

  • Small GTP-binding protein
  • Rab5
  • Rab GDI
  • Membrane traffic
Affiliation Not a UNIGE publication
Citation (ISO format)
STEELE-MORTIMER, Olivia, GRUENBERG, Jean, CLAGUE, Michael J. Phosphorylation of GDI and membrane cycling of rab proteins. In: FEBS letters, 1993, vol. 329, n° 3, p. 313–318. doi: 10.1016/0014-5793(93)80244-O
Main files (1)
Article (Published version)
ISSN of the journal0014-5793

Technical informations

Creation05/26/2016 3:13:00 PM
First validation05/26/2016 3:13:00 PM
Update time03/15/2023 12:25:31 AM
Status update03/15/2023 12:25:30 AM
Last indexation01/16/2024 8:58:51 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack