Scientific Article
previous document  unige:84273  next document
add to browser collection

Phosphorylation of GDI and membrane cycling of rab proteins

Steele-Mortimer, Olivia
Clague, Michael J.
Published in FEBS Letters. 1993, vol. 329, no. 3, p. 313-318
Abstract Membrane transport is known to be regulated by protein phosphorylation and by small GTPases of the rab family. Using specific antibodies, we have identified a 55 kDa phosphorylated protein which co-immunoprecipitated with the cytosolic forms of rab5 and other rab proteins. We demonstrate, on the basis of its mobility in two-dimensional electrophoresis gels and its immunological properties, that this protein is rab GDI (p55/GDI). We also found that, a minor fraction of p55/GDI is membrane associated, but, whilst also complexed with rab proteins, it is not phosphorylated. On the basis of these data we suggest that the cycling of rab proteins between membranes and cytosol is regulated by phosphorylation of p55/GDI.
Keywords Small GTP-binding proteinRab5Rab GDIMembrane traffic
Full text
(ISO format)
STEELE-MORTIMER, Olivia, GRUENBERG, Jean, CLAGUE, Michael J. Phosphorylation of GDI and membrane cycling of rab proteins. In: FEBS Letters, 1993, vol. 329, n° 3, p. 313-318.

123 hits

0 download


Deposited on : 2016-06-07

Export document
Format :
Citation style :