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Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations |
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Published in | Journal of pharmaceutical and biomedical analysis. 1998, vol. 17, no. 6-7, p. 1167-76 | |
Abstract | The simultaneous determination of atropine and scopolamine derivatives, which have similar structures, was investigated by using capillary zone electrophoresis. The effects of buffer pH, buffer concentration and hydroxypropyl-beta-cyclodextrin concentration on migration time and resolution of the investigated compounds were systematically studied. The selected electrophoretic buffer consisted of a 80 mM sodium citrate pH 2.5, containing 2.5 mM hydroxypropyl-beta-cyclodextrin as the complexing agent. Quantitative analysis was validated by testing the reproducibility of the method, giving a relative standard deviation less than 1 and 2% for the intermediate precision of migration times and peak area ratios, respectively. The linearity of the method was assessed between 50 and 150% of the theoretical content (coefficient of correlation greater than 0.99). The proposed method was found to be suitable and accurate for the determination of these basic drugs in pharmaceutical preparations. | |
Keywords | Atropine Derivatives/analysis — Cyclodextrins/chemistry — Drug Contamination — Electrophoresis, Capillary/instrumentation/methods — Hydrogen-Ion Concentration — Reproducibility of Results — Scopolamine Derivatives/analysis — Sensitivity and Specificity — Time Factors — Beta-Cyclodextrins | |
Identifiers | PMID: 9884207 | |
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Citation (ISO format) | CHERKAOUI, Samir et al. Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations. In: Journal of pharmaceutical and biomedical analysis, 1998, vol. 17, n° 6-7, p. 1167-76. doi: 10.1016/S0731-7085(98)00083-1 https://archive-ouverte.unige.ch/unige:8426 |