en
Scientific article
English

Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations

Published inJournal of pharmaceutical and biomedical analysis, vol. 17, no. 6-7, p. 1167-1176
Publication date1998
Abstract

The simultaneous determination of atropine and scopolamine derivatives, which have similar structures, was investigated by using capillary zone electrophoresis. The effects of buffer pH, buffer concentration and hydroxypropyl-beta-cyclodextrin concentration on migration time and resolution of the investigated compounds were systematically studied. The selected electrophoretic buffer consisted of a 80 mM sodium citrate pH 2.5, containing 2.5 mM hydroxypropyl-beta-cyclodextrin as the complexing agent. Quantitative analysis was validated by testing the reproducibility of the method, giving a relative standard deviation less than 1 and 2% for the intermediate precision of migration times and peak area ratios, respectively. The linearity of the method was assessed between 50 and 150% of the theoretical content (coefficient of correlation greater than 0.99). The proposed method was found to be suitable and accurate for the determination of these basic drugs in pharmaceutical preparations.

Keywords
  • Atropine Derivatives/analysis
  • Cyclodextrins/chemistry
  • Drug Contamination
  • Electrophoresis, Capillary/instrumentation/methods
  • Hydrogen-Ion Concentration
  • Reproducibility of Results
  • Scopolamine Derivatives/analysis
  • Sensitivity and Specificity
  • Time Factors
  • Beta-Cyclodextrins
Citation (ISO format)
CHERKAOUI, Samir et al. Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations. In: Journal of pharmaceutical and biomedical analysis, 1998, vol. 17, n° 6-7, p. 1167–1176. doi: 10.1016/S0731-7085(98)00083-1
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Article (Published version)
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Identifiers
ISSN of the journal0731-7085
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