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Title

Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations
Authors
Cherkaoui, Samir
Mateus, Lidia
Christen, Philippe
Veuthey, Jean-Luc
Published in Journal of pharmaceutical and biomedical analysis. 1998, vol. 17, no. 6-7, p. 1167-76
Abstract The simultaneous determination of atropine and scopolamine derivatives, which have similar structures, was investigated by using capillary zone electrophoresis. The effects of buffer pH, buffer concentration and hydroxypropyl-beta-cyclodextrin concentration on migration time and resolution of the investigated compounds were systematically studied. The selected electrophoretic buffer consisted of a 80 mM sodium citrate pH 2.5, containing 2.5 mM hydroxypropyl-beta-cyclodextrin as the complexing agent. Quantitative analysis was validated by testing the reproducibility of the method, giving a relative standard deviation less than 1 and 2% for the intermediate precision of migration times and peak area ratios, respectively. The linearity of the method was assessed between 50 and 150% of the theoretical content (coefficient of correlation greater than 0.99). The proposed method was found to be suitable and accurate for the determination of these basic drugs in pharmaceutical preparations.
Keywords Atropine Derivatives/analysis — Cyclodextrins/chemistry — Drug Contamination — Electrophoresis, Capillary/instrumentation/methods — Hydrogen-Ion Concentration — Reproducibility of Results — Scopolamine Derivatives/analysis — Sensitivity and Specificity — Time Factors — Beta-Cyclodextrins
Identifiers
PMID: 9884207
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Article (Published version) (149 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		CHERKAOUI, Samir et al. Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations. In: Journal of pharmaceutical and biomedical analysis, 1998, vol. 17, n° 6-7, p. 1167-76. doi: 10.1016/S0731-7085(98)00083-1 https://archive-ouverte.unige.ch/unige:8426

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Deposited on : 2010-06-24

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