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Scientific article
English

CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells

Published inCurrent gene therapy, vol. 15, no. 5, p. 447-459
Publication date2015
Abstract

Polarized airway epithelial cell cultures modelling Cystic Fibrosis Transmembrane conductance Regulator (CFTR) defect are crucial for CF and biomedical research. RNA interference has proven its value to generate knockdown models for various pathologies. More recently, genome editing using CRISPR-Cas9 artificial endonuclease was a valuable addition to the toolbox of gene inactivation.

Keywords
  • CFTR
  • CRISPR-Cas9
  • Cystic fibrosis
  • Lentiviral vector
  • Primary cells
  • RNA interference
Citation (ISO format)
BELLEC, Jessica et al. CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells. In: Current gene therapy, 2015, vol. 15, n° 5, p. 447–459. doi: 10.2174/1566523215666150812115939
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1566-5232
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7downloads

Technical informations

Creation06/06/2016 11:18:00 AM
First validation06/06/2016 11:18:00 AM
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