CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells

Bellec, Jessica; Bacchetta, Marc; Losa, Davide; Anegon, Ignacio; Chanson, Marc; Nguyen, Tuan Huy

doi:10.2174/1566523215666150812115939

Scientific article

English

CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells

ContributorsBellec, Jessica; Bacchetta, Marc; Losa, Davide; Anegon, Ignacio; Chanson, Marc

; Nguyen, Tuan Huy

Published inCurrent gene therapy, vol. 15, no. 5, p. 447-459

Publication date2015

Abstract

Polarized airway epithelial cell cultures modelling Cystic Fibrosis Transmembrane conductance Regulator (CFTR) defect are crucial for CF and biomedical research. RNA interference has proven its value to generate knockdown models for various pathologies. More recently, genome editing using CRISPR-Cas9 artificial endonuclease was a valuable addition to the toolbox of gene inactivation.

Keywords

CFTR
CRISPR-Cas9
Cystic fibrosis
Lentiviral vector
Primary cells
RNA interference

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Research groups

Mucoviscidose et jonctions GAP (229)

Citation (ISO format)

BELLEC, Jessica et al. CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells. In: Current gene therapy, 2015, vol. 15, n° 5, p. 447–459. doi: 10.2174/1566523215666150812115939

Article (Published version)

Identifiers

PID : unige:84234
DOI : 10.2174/1566523215666150812115939
PMID : 26264708

Journal ISSN1566-5232

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CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells

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