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An artificial PPR scaffold for programmable RNA recognition

Filipovska, Aleksandra
Chia, Tiongsun
Lingford, James P
Razif, Muhammad F M
Rackham, Oliver
Published in Nature communications. 2014, vol. 5, 5729
Abstract Pentatricopeptide repeat (PPR) proteins control diverse aspects of RNA metabolism in eukaryotic cells. Although recent computational and structural studies have provided insights into RNA recognition by PPR proteins, their highly insoluble nature and inconsistencies between predicted and observed modes of RNA binding have restricted our understanding of their biological functions and their use as tools. Here we use a consensus design strategy to create artificial PPR domains that are structurally robust and can be programmed for sequence-specific RNA binding. The atomic structures of these artificial PPR domains elucidate the structural basis for their stability and modelling of RNA-protein interactions provides mechanistic insights into the importance of RNA-binding residues and suggests modes of PPR-RNA association. The modular mode of RNA binding by PPR proteins holds great promise for the engineering of new tools to target RNA and to understand the mechanisms of gene regulation by natural PPR proteins.
Keywords Amino Acid MotifsArabidopsis/genetics/metabolismBinding SitesGene Expression RegulationHumansMitochondrial Proteins/chemistry/genetics/metabolismModels, MolecularMolecular Sequence DataProtein BindingProtein EngineeringProtein StabilityProtein Structure, TertiaryRNA/chemistry/genetics/metabolismRNA-Binding Proteins/chemistry/genetics/metabolismRecombinant Proteins/chemistry/genetics/metabolism
PMID: 25517350
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COQUILLE, Sandrine Claire et al. An artificial PPR scaffold for programmable RNA recognition. In: Nature communications, 2014, vol. 5, p. 5729. doi: 10.1038/ncomms6729 https://archive-ouverte.unige.ch/unige:84233

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Deposited on : 2016-06-06

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