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Evidence for Differential Glycosylation of Human Trophoblast Cell Types

Chen, Qiushi
Pang, Poh-Choo
Longtine, Mark S
Schust, Danny J
Haslam, Stuart M
Blois, Sandra M
Dell, Anne
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Published in Molecular and Cellular Proteomics. 2016, vol. 15, no. 6, p. 1857-1866
Abstract Human placental villi are surfaced by the syncytiotrophoblast (STB), with a layer of cytotrophoblasts (CTB) positioned just beneath the STB. STB in normal term pregnancies is exposed to maternal immune cells in the placental intervillous space. Extravillous cytotrophoblasts (EVT) invade the decidua and spiral arteries, where they act in conjunction with natural killer (NK) cells to convert the spiral arteries into flaccid conduits for maternal blood that support a 3-4 fold increase in the rate of maternal blood flow into the placental intervillous space. The distinct functional roles of these trophoblast subtypes during pregnancy suggest that they could be differentially glycosylated. Glycomic analysis of these placental cells has revealed the expression of elevated levels of biantennary N-glycans in STB and CTB, with the majority bearing a bisecting GlcNAc. N-glycans terminated with polylactosamine extensions were also detected at low levels. A subset of the N-glycans linked to these trophoblasts were sialylated, primarily with terminal NeuAcα2-3Gal sequences. EVT were decorated with the same N-glycans as STB and CTB, except in different proportions. The level of bisecting type N-glycans in EVT was reduced, but the level of N-glycans decorated with polylactosamine sequences was substantially elevated compared to the other types of trophoblasts. The level of triantennary and tetraantennary N-glycans was also elevated in EVT. The sialylated N-glycans derived from EVT were completely susceptible to an α2-3 specific neuraminidase (sialidase S). The possibility exists that the N-glycans associated with these different trophoblast subpopulations could act as functional groups. These potential relationships will be discussed.
PMID: 26929217
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Research group Laboratoire de biologie des tumeurs gynécologiques et du développement (927)
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CHEN, Qiushi et al. Evidence for Differential Glycosylation of Human Trophoblast Cell Types. In: Molecular and Cellular Proteomics, 2016, vol. 15, n° 6, p. 1857-1866. https://archive-ouverte.unige.ch/unige:84150

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Deposited on : 2016-06-02

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