Scientific article

Fat and Sucrose Intake Induces Obesity-Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats

Published inJournal of bone and mineral research, vol. 31, no. 1, p. 98-115
Publication date2016

Metabolic and bone effects were investigated in growing (G, n¼45) and mature (M, n¼45) rats fed a high-fat/high-sucrose diet(HFS)isocaloric to the chow diet of controls (C, n¼30 per group). At week 19, a subset of 15 rats in each group (HFS or C, at both ages)was analyzed. Then one-half of the remaining 30 HFS rats in each groups continued HFS and one-half were shifted to C until week 27. Although no serum or bone marrow inflammation was seen, HFS increased visceral fat, serum leptin and insulin at week 19 and induced further alterations in lipid profile, serum adiponectin, and TGFb1, TIMP1, MMP2, and MMP9, suggesting a prediabetic phenotype and cardiovascular dysfunction at week 27 more pronounced in M than G. These events were associated with dramatic reduction of osteoclastic and osteoid surfaces with accelerated mineralizing surfaces in both HFS age groups. Mineral metabolism and its major regulators were disturbed, leading to hyperphosphatemia and hypocalcemia. These changes were associated with bone alterations in the weight-bearing tibia, not in the non-weight-bearing vertebra. Indeed in fat rats, tibia trabecular bone accrual increased in G whereas loss of trabecular bone inMwas alleviated. At diaphysis cortical porosity increased in G and even more inMat week 27. After the diet switch, metabolic and bone cellular disturbances fully reversed in G, but not in M. Trabecular benefit of the obese was preserved in both age groups and inMthe age-related bone loss was even lighter after the diet switch than in prolonged HFS. At the diaphysis, cortical porosity normalized in G but not in M. Hypocalcemia in G and M was irreversible. Thus, the mild metabolic syndrome induced by isocaloric HFS is able to alter bone cellular activities and mineral metabolism, reinforce trabecular bone, and affect cortical bone porosity in an irreversible manner in older rats. © 2015 American Society for Bone and Mineral Research.

  • Obesity
  • Diabetes
  • Calcium-Phosphate metabolism
  • Histomorphometry
  • BOone Strength
Citation (ISO format)
LAVET, Cedric et al. Fat and Sucrose Intake Induces Obesity-Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats. In: Journal of bone and mineral research, 2016, vol. 31, n° 1, p. 98–115. doi: 10.1002/jbmr.2596
Main files (1)
Article (Published version)
ISSN of the journal0884-0431

Technical informations

Creation01/19/2016 10:39:00 AM
First validation01/19/2016 10:39:00 AM
Update time03/15/2023 12:22:53 AM
Status update03/15/2023 12:22:53 AM
Last indexation01/16/2024 8:52:25 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack