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Titles listHIV-1 replication in dendritic cells occurs through a tetraspanin-containing compartment enriched in AP-3
Titles listHIV-1 replication in dendritic cells occurs through a tetraspanin-containing compartment enriched in AP-3
Scientific Article
unige:834 
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HIV-1 replication in dendritic cells occurs through a tetraspanin-containing compartment enriched in AP-3 |
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| Authors | ||
| Published in | Traffic. 2008, vol. 9, no. 2, p. 200-14 | |
| Abstract | Dendritic cells (DC) are crucial components of the early events of HIV infection. Dendritic cells capture and internalize HIV at mucosal surfaces and efficiently transfer the virus to CD4+ T cells in trans through infectious synapses (trans-infection pathway). Alternatively, HIV-1 replicates in DC (R5-HIV-1) (cis-infection pathway). Here, we analyzed HIV trafficking in DC during the trans-infection pathway as well as the cis-infection pathway. Confocal immunofluorescence microscopy demonstrated that after capture by DC, R5-HIV-1 and HIV-1 pseudotyped with vesicular stomatitis virus protein G colocalized in a viral compartment enriched in tetraspanins including CD81, CD82 and CD9, although at different levels, indicating a role of the viral envelope in targeting to the tetraspanin-rich compartment. Replication of R5-HIV-1 in DC (cis-infection pathway) also led to the accumulation, in an envelope-independent manner, of mature viral particles in a tetraspanin-rich compartment. A fraction of the HIV-1-containing compartments appeared directly accessible from the cell surface. In sharp contrast with the trans-infection pathway, the delta-subunit of the adaptor protein 3 (AP-3) complex was enriched on the HIV-1-containing compartment during R5-HIV-1 replication in DC (cis-infection pathway). Downregulation of AP-3 delta-adaptin reduced significantly viral particle release from HIV-1-infected DC. Together, these studies demonstrate a role for AP-3 in HIV replication in a tetraspanin-rich compartment in DC and contribute to the elucidation of the trafficking pathways required for DC-T cell transfer of HIV-1 infection, a critical step during the early events of HIV infection. | |
| Keywords | Adaptor Protein Complex 3/physiology — Adaptor Protein Complex delta Subunits/physiology — Antigens CD/analysis/metabolism — Biological Transport/physiology — CD4-Positive T-Lymphocytes/virology — Cell Communication/physiology — Coculture Techniques — Cytoplasmic Vesicles/chemistry/virology — Dendritic Cells/metabolism/virology — HIV Antigens/analysis — HIV-1/physiology — HLA-DR Antigens/analysis — Humans — Kangai-1 Protein/analysis — Lysosome-Associated Membrane Glycoproteins/analysis — Membrane Glycoproteins — Membrane Proteins/analysis/metabolism — Platelet Membrane Glycoproteins/analysis — RNA Small Interfering/genetics — Viral Envelope Proteins/physiology — Virion/chemistry/physiology — Virus Replication/physiology — Gag Gene Products Human Immunodeficiency Virus/analysis | |
| Identifiers | PMID: 18034776 | |
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| Citation (ISO format) | GARCIA, Eduardo, NIKOLIC, Damjan, PIGUET, Vincent. HIV-1 replication in dendritic cells occurs through a tetraspanin-containing compartment enriched in AP-3. In: Traffic, 2008, vol. 9, n° 2, p. 200-14. doi: 10.1111/j.1600-0854.2007.00678.x https://archive-ouverte.unige.ch/unige:834 |
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