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Scientific article
English

Neuronal specificity of the α7 nicotinic acetylcholine receptor promoter develops during morphogenesis of the central nervous system

Published inEMBO journal, vol. 11, no. 12, p. 4529-4538
Publication date1992
Abstract

A transient transfection assay has been developed to analyse promoter activity in neuronal cells freshly dissociated from the chick central nervous system. The assay enabled us to identify cis-acting regulatory elements within the 5'-flanking region of the alpha 7 nicotinic acetylcholine receptor gene. In differentiated retina, regulatory elements direct reporter gene expression to a small subset of neurons which has been identified as ganglion cells, i.e. to the population of neurons in which alpha 7 transcripts were localized by in situ hybridization. However, these promoter elements exhibit ubiquitous activity in undifferentiated neural cells and in mesodermal stem cells. Our study supports the idea that alpha 7 regulatory elements acquire their neuronal specificity in the course of embryogenesis.

Keywords
  • Acetylcholine receptor
  • Neurogenesis
  • Neuron transfection
  • Neuronal promoter
Citation (ISO format)
MATTER-SADZINSKI, Lidia et al. Neuronal specificity of the α7 nicotinic acetylcholine receptor promoter develops during morphogenesis of the central nervous system. In: EMBO journal, 1992, vol. 11, n° 12, p. 4529–4538.
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Article (Published version)
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Identifiers
  • PID : unige:83300
ISSN of the journal0261-4189
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