Scientific Article
previous document  unige:82753  next document
add to browser collection
Title

The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo

Authors
Bernet-Camard, Marie-Françoise
Liévin, Vanessa
Brassart, Dominique
Servin, Alain L.
Hudault, Sylvie
Published in Applied and Environmental Microbiology. 1997, vol. 63, no. 7, p. 2747-2753
Abstract The adhering human Lactobacillus acidophilus strain LA1 inhibits the cell association and cell invasion of enteropathogens in cultured human intestinal Caco-2 cells (M. F. Bernet, D. Brassard, J. R. Neeser, and A. L. Servin, Gut 35:483-489, 1994). Here, we demonstrate that strain LA1 developed its antibacterial activity in conventional or germ-free mouse models orally infected by Salmonella typhimurium. We present evidence that the spent culture supernatant of strain LA1 (LA1-SCS) contained antibacterial components active against S. typhimurium infecting the cultured human intestinal Caco-2 cells. The LA1-SCS antibacterial activity was observed in vitro against a wide range of gram-negative and gram-positive pathogens, such as Staphylococcus aureus, Listeria monocytogenes, S. typhimurium, Shigella flexneri, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterobacter cloacae. By contrast, no activity was observed against species of the normal gut flora, such as lactobacilli and bifidobacteria. The LA1-SCS antibacterial activity was insensitive to proteases and independent of lactic acid production.
Keywords AnimalsAnti-Bacterial Agents/metabolism/pharmacologyAntibiosisCaco-2 CellsCellsCulturedEndopeptidases/pharmacologyFemaleGerm-Free LifeGram-Negative Bacteria/drug effectsGram-Positive Bacteria/drug effectsHumansLactic Acid/metabolismLactobacillus acidophilus/metabolismMiceMiceInbred C3HMicrobial Sensitivity TestsMicroscopyElectronSalmonella InfectionsAnimal/microbiologySalmonella typhimurium/ultrastructure
Identifiers
PMID: 9212421
Full text
Article (Published version) (203 Kb) - public document Free access
Citation
(ISO format)
BERNET-CAMARD, Marie-Françoise et al. The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo. In: Applied and Environmental Microbiology, 1997, vol. 63, n° 7, p. 2747-2753. https://archive-ouverte.unige.ch/unige:82753

86 hits

31 downloads

Update

Deposited on : 2016-04-13

Export document
Format :
Citation style :