UNIGE document Scientific Article
previous document  unige:82424  next document
add to browser collection

Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum

de Barsy, Marie
Greub, Gilbert
Published in ISME Journal. 2016, vol. 10, no. 9, p. 2129-2144
Abstract Like other obligate intracellular bacteria, the Chlamydiae feature a compact regulatory genome that remains uncharted owing to poor genetic tractability. Exploiting the reduced number of transcription factors (TFs) encoded in the chlamydial (pan-)genome as a model for TF control supporting the intracellular lifestyle, we determined the conserved landscape of TF specificities by ChIP-Seq (chromatin immunoprecipitation-sequencing) in the chlamydial pathogen Waddlia chondrophila. Among 10 conserved TFs, Euo emerged as a master TF targeting >100 promoters through conserved residues in a DNA excisionase-like winged helix-turn-helix-like (wHTH) fold. Minimal target (Euo) boxes were found in conserved developmentally-regulated genes governing vertical genome transmission (cytokinesis and DNA replication) and genome plasticity (transposases). Our ChIP-Seq analysis with intracellular bacteria not only reveals that global TF regulation is maintained in the reduced regulatory genomes of Chlamydiae, but also predicts that master TFs interpret genomic information in the obligate intracellular α-proteobacteria, including the rickettsiae, from which modern day mitochondria evolved.The ISME Journal advance online publication, 8 March 2016; doi:10.1038/ismej.2016.23.
PMID: 26953603
Full text
Article (Published version) (3.1 MB) - public document Free access
Research group Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
(ISO format)
DE BARSY, Marie et al. Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum. In: ISME Journal, 2016, vol. 10, n° 9, p. 2129-2144. https://archive-ouverte.unige.ch/unige:82424

208 hits



Deposited on : 2016-04-06

Export document
Format :
Citation style :