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Scientific article
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English

Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum

Published inThe ISME journal, vol. 10, no. 9, p. 2129-2144
Publication date2016
Abstract

Like other obligate intracellular bacteria, the Chlamydiae feature a compact regulatory genome that remains uncharted owing to poor genetic tractability. Exploiting the reduced number of transcription factors (TFs) encoded in the chlamydial (pan-)genome as a model for TF control supporting the intracellular lifestyle, we determined the conserved landscape of TF specificities by ChIP-Seq (chromatin immunoprecipitation-sequencing) in the chlamydial pathogen Waddlia chondrophila. Among 10 conserved TFs, Euo emerged as a master TF targeting >100 promoters through conserved residues in a DNA excisionase-like winged helix-turn-helix-like (wHTH) fold. Minimal target (Euo) boxes were found in conserved developmentally-regulated genes governing vertical genome transmission (cytokinesis and DNA replication) and genome plasticity (transposases). Our ChIP-Seq analysis with intracellular bacteria not only reveals that global TF regulation is maintained in the reduced regulatory genomes of Chlamydiae, but also predicts that master TFs interpret genomic information in the obligate intracellular α-proteobacteria, including the rickettsiae, from which modern day mitochondria evolved.The ISME Journal advance online publication, 8 March 2016; doi:10.1038/ismej.2016.23.

Citation (ISO format)
DE BARSY, Marie et al. Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum. In: The ISME journal, 2016, vol. 10, n° 9, p. 2129–2144. doi: 10.1038/ismej.2016.23
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ISSN of the journal1751-7362
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