Proteolytic Cleavage of a Spectrin-Related Protein by Calcium-Dependent Protease in Neurospora crassa

Cotado Sampayo, Marta; Ojha, Mukti; Ortega Perez, Ruben Dario; Chappuis, Marie-Louise; Barja, François

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Title		Proteolytic Cleavage of a Spectrin-Related Protein by Calcium-Dependent Protease in Neurospora crassa
Authors		Cotado Sampayo, Marta Ojha, Mukti Ortega Perez, Ruben Dario Chappuis, Marie-Louise Barja, François
Published in		Current Microbiology. 2006, vol. 53, no. 4, p. 311-316
Abstract		To investigate the functional significance of a cytoskeletal spectrin-like protein, we studied its localization pattern in Neurospora crassa and sought the answer to whether it is a substrate for another apically localized protein, the calcium-dependent protease (CDP II). Immunoblots of crude extracts from exponentially growing mycelia, separated by one- and two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis using antichicken α/β-spectrin antibodies, revealed a single band of approximately relative mass (Mr) 100 kDa with an isoeletric point (pI) in the range of 6.5 to 7.0. Despite rigorous efforts, we could not confirm the presence of an Mr 240- to 220-kDa spectrin-like protein in N. crassa. The immunofluorescence- and immunogold-labeling Mr 100-kDa protein showed its predominance along the plasma membrane of the conidia during the swelling phase of germination. In contrast, in the germ tubes and the growing hyphae, the localization was polarized and concentrated mainly in the apical region. The in vitro proteolysis experiments showed that indeed this protein is a preferred substrate of CDP II which is, as mentioned previously, also localized in the apical regions of the hyphae. These results indicate a putative functional relationship between these two proteins (spectrin-like protein and CDP II) in the dynamics of tip growth.
Identifiers		DOI: 10.1007/s00284-006-0057-y
Full text		Article (Published version) (199 Kb) - Limited access to UNIGE Other version: http://link.springer.com/10.1007/s00284-006-0057-y
Structures		Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté des sciences / Section de chimie et biochimie
Citation (ISO format)		COTADO SAMPAYO, Marta et al. Proteolytic Cleavage of a Spectrin-Related Protein by Calcium-Dependent Protease in Neurospora crassa. In: Current Microbiology, 2006, vol. 53, n° 4, p. 311-316. https://archive-ouverte.unige.ch/unige:82349