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Occurrence of Antibiotic Resistance Genes and Bacterial Markers in a Tropical River Receiving Hospital and Urban Wastewaters

Mulaji, Crispin Kyela
Otamonga, Jean-Paul
Mpiana, Pius Tshimankinda
Mubedi, Josué Ilunga
Prabakar, Kandasamy
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Published in PLOS ONE. 2016, vol. 11, no. 2, e0149211
Abstract The occurrence of emerging biological contaminants including antibiotic resistance genes (ARGs) and Faecal Indicator Bacteria (FIB) is still little investigated in developing countries under tropical conditions. In this study, the total bacterial load, the abundance of FIB (E. coli and Enterococcus spp. (ENT)), Pseudomonas spp. and ARGs (blaTEM, blaCTX-M, blaSHV, blaNDM and aadA) were quantified using quantitative PCR in the total DNA extracted from the sediments recovered from hospital outlet pipes (HOP) and the Cauvery River Basin (CRB), Tiruchirappalli, Tamil Nadu, India. The abundance of bacterial marker genes were 120, 104 and 89 fold higher for the E. coli, Enterococcus spp. and Pseudomonas spp., respectively at HOP when compared with CRB. The ARGs aadA and blaTEM were most frequently detected in higher concentration than other ARGs at all the sampling sites. The ARGs blaSHV and blaNDM were identified in CRB sediments contaminated by hospital and urban wastewaters. The ARGs abundance strongly correlated (r ≥ 0.36, p < 0.05, n = 45) with total bacterial load and E. coli in the sediments, indicating a common origin and extant source of contamination. Tropical aquatic ecosystems receiving wastewaters can act as reservoir of ARGs, which could potentially be transferred to susceptible bacterial pathogens at these sites.
Keywords Hospital and urban wastewaterTropical RiverSedimentAntibiotic resistance genesß-lactamasesCauvery River-India
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Research groups Environmental Biogeochemistry and Ecotoxicology
Environmental Physical Chemistry
FORE9 Environmental microbiology
ISE Biodiversité
ISE Pôle Sciences
Limnology and Environmental Geology
Microbial Ecology
Project FNS: grant no. 31003A_150163/1
(ISO format)
DEVARAJAN, Naresh et al. Occurrence of Antibiotic Resistance Genes and Bacterial Markers in a Tropical River Receiving Hospital and Urban Wastewaters. In: PLOS ONE, 2016, vol. 11, n° 2, p. e0149211. doi: 10.1371/journal.pone.0149211 https://archive-ouverte.unige.ch/unige:81095

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Deposited on : 2016-02-29

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