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Mass spectrometry-based selectivity and sensitivity enhancement for the quantification of protein expression changes in anti-viral response mechanisms

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Defense Thèse de doctorat : Univ. Genève, 2016 - Sc. 4885 - 2016/01/25
Abstract Multiple Reaction Monitoring (MRM) is a highly sensitive and selective mass spectrometry technique for peptide quantification in proteomics. The development of an MRM assay method is critical for highly rapid, accurate, and reproducible measurements. We developed an automated workflow to generate optimal MRM transitions based on empirical synthetic peptide libraries produced by flow injection analysis using in-house developed software called MRMOptimizer. Furthermore, the evaluation of alternative techniques to MRM, such as multiple reaction monitoring cubed (MRM³) and differential ion mobility mass spectrometry (DMS), in terms of selectivity and sensitivity was an essential part of this thesis. Finally, we applied our optimized MRM assay method to investigate human monocyte-derived dendritic cell (MDDC) protein expression during the early HIV-1 infection state.
Keywords ProteomicsMultiple Reaction MonitoringMultiple Reaction Monitoring CubedDifferential Ion Mobility SpectrometryAnti-viral ResponseDendritic cells
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URN: urn:nbn:ch:unige-809307
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Thesis (7.9 MB) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Spectrométrie de masse du vivant
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ALGHANEM, Bandar. Mass spectrometry-based selectivity and sensitivity enhancement for the quantification of protein expression changes in anti-viral response mechanisms. Université de Genève. Thèse, 2016. https://archive-ouverte.unige.ch/unige:80930

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Deposited on : 2016-02-24

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