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Master
English

Correlation between DNA sequence, nucleosome stability and transcriptional output

Contributors

Raykov, Lyudmil

Directors

Shore, David M.

Master program title

Genetics, Development and Evolution

Defense date

2016

Abstract

The role of the DNA sequence on the nucleosome dynamics remains poorly understood, therefore t h e main biological question tackled by this project is: Does the DNA sequence contribute to nucleosome stability? To answer this question, in the following work we will generate a pool of plasmids with at least one million diferent yeast ribosomal protein gene RPS11A promoter variants, each one with altered, random 145 bp DNA sequence underneath the -1 FN, immediately upstream of an fuorescence reporter ORF. This library will then be transformed into budding yeast and the transformants will be pooled and sorted out according to the transcriptional output by fow cytometry. The DNA sequences underneath the -1 FNs will then be amplifed, subject to pairedend sequencing, and screened for motifs correlated with expression.

Keywords

  • Fragile nucleosome
  • Transcription regulation

UNIGE affiliation entities

UNIGE research groups

Funding

  • Autre - Master project
  • Autre - Master project

Citation (ISO format)

RAYKOV, Lyudmil. Correlation between DNA sequence, nucleosome stability and transcriptional output. Master, 2016.

Main files (1)

Master thesis

Identifiers

  • PID : unige:80704
163views
15downloads

Technical informations

Creation

12/02/2016 12:28:00

First validation

12/02/2016 12:28:00

Update

15/03/2023 00:08:24

Status update

15/03/2023 00:08:24

Last indexation

31/10/2024 02:45:11
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