en
Master
English

Correlation between DNA sequence, nucleosome stability and transcriptional output

ContributorsRaykov, Lyudmil
Master program titleGenetics, Development and Evolution
Defense date2016
Abstract

The role of the DNA sequence on the nucleosome dynamics remains poorly understood, therefore t h e main biological question tackled by this project is: Does the DNA sequence contribute to nucleosome stability? To answer this question, in the following work we will generate a pool of plasmids with at least one million diferent yeast ribosomal protein gene RPS11A promoter variants, each one with altered, random 145 bp DNA sequence underneath the -1 FN, immediately upstream of an fuorescence reporter ORF. This library will then be transformed into budding yeast and the transformants will be pooled and sorted out according to the transcriptional output by fow cytometry. The DNA sequences underneath the -1 FNs will then be amplifed, subject to pairedend sequencing, and screened for motifs correlated with expression.

eng
Keywords
  • Fragile nucleosome
  • Transcription regulation
Research group
Funding
  • Autre - Master project
  • Autre - Master project
Citation (ISO format)
RAYKOV, Lyudmil. Correlation between DNA sequence, nucleosome stability and transcriptional output. 2016.
Main files (1)
Master thesis
accessLevelRestricted
Identifiers
  • PID : unige:80704
73views
15downloads

Technical informations

Creation02/12/2016 12:28:00 PM
First validation02/12/2016 12:28:00 PM
Update time03/15/2023 12:08:24 AM
Status update03/15/2023 12:08:24 AM
Last indexation01/29/2024 8:41:23 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack