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Revealing the importance of the mRNA export factor Yra1 in maintaining genome integrity in the yeast Saccharomyces cerevisiae

ContributorsZihlmann, Audrey
Master program titleMaster en biologie orientation libre
Defense date2016
Abstract

Transcription and export of mRNA are two essential processes that drive gene expression in all eukaryotic cells. The TREX complex links these processes by mediating the co-transcriptional recruitment of the export machinery to transcribing genes and nascent mRNAs. Although the TREX complex is best known for its function in transcription and export, it has also been linked to the maintenance of actively transcribed loci and therefore to genome integrity. Yra1, a component of the TREX complex, is ubiquitinated by a SUMO-trageted E3 ligase (STUbL) composed of two subunits, Slx5 and Slx8, which are known to have an effect on genome stability. Yra1 ubiquitination by Slx5/Slx8 is not linked to its degradation or export functions, suggesting that Yra1 ubiquitination could be important to maintain genome integrity. Maintaining genome integrity is not only to keep DNA sequences intact throughout replication or following damages, but also to ensure that sister chromatids separate and segregate correctly during mitosis. Here, we show that yra1 mutants exhibit spindle orientation defects and aberrant spindle morphologies, suggesting that Yra1 could play a role in genome integrity by ensuring proper spindle orientation during cell cycle. Interestingly, we also show that yra1 mutants increase Rad52 foci, providing additional indication of a potential role in genome stability. Future work will focus on understanding the molecular basis of these phenotypes and whether Yra1 plays a direct role in these cell-cycle dependent processes.

eng
Keywords
  • Yra1
  • TREX complexe
  • Genome integrity
  • Spindle orientation
  • DSB
  • Genome instability
Research group
Citation (ISO format)
ZIHLMANN, Audrey. Revealing the importance of the mRNA export factor Yra1 in maintaining genome integrity in the yeast Saccharomyces cerevisiae. 2016.
Main files (1)
Master thesis
accessLevelRestricted
Identifiers
  • PID : unige:80639
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Creation02/10/2016 1:28:00 PM
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