Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn<sup>2+</sup>cations in cartilage and meniscus calcifications

Dessombz, Arnaud; Nguyen, Christelle; Ea, Hang-Korng; Rouzière, Stephan; Foy, Eddy; Hannouche, Didier; Réguer, Solene; Picca, Frederic-Emmanuel; Thiaudière, Dominique; Lioté, Frédéric; Daudon, Michel; Bazin, Dominique

doi:10.1016/j.jtemb.2013.02.001

Scientific article

English

Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn²⁺cations in cartilage and meniscus calcifications

Contributors

Dessombz, Arnaud; Nguyen, Christelle; Ea, Hang-Korng; Rouzière, Stephan; Foy, Eddy; Hannouche, Didier

; Réguer, Solene; Picca, Frederic-Emmanuel; Thiaudière, Dominique; Lioté, Frédéric; Daudon, Michel; Bazin, Dominique

Published in

Journal of trace elements in medicine and biology, vol. 27, no. 4, p. 326-333

Publication date

2013-10

Abstract

We aimed to examine the presence of Zn, a trace element, in osteoarthritis (OA) cartilage and meniscus from patients undergoing total knee joint replacement for primary OA. We mapped Ca²⁺ and Zn²⁺ at the mesoscopic scale by X-ray fluorescence microanalysis (μX-ray) to determine the spatial distribution of the 2 elements in cartilage, μX-ray absorption near edge structure spectroscopy to identify the Zn species, and μX-ray diffraction to determine the chemical nature of the calcification. Fourier transform infrared spectroscopy was used to determine the chemical composition of cartilage and meniscus. Ca²⁺ showed a heterogeneous spatial distribution corresponding to the calcifications within cartilage (or meniscus) or at their surface. At least 2 Zn²⁺ species were present: the first may correspond to Zn embedded in protein (different Zn metalloproteins are known to prevent calcification in biological tissues), and the second may be associated with a Zn trap in or at the surface of the calcification. Calcification present in OA cartilage may significantly modify the spatial distribution of Zn; part of the Zn may be trapped in the calcification and may alter the associated biological function of Zn metalloproteins.

Keywords

Osteoarthritis
Ca phosphate apatites
Ca pyrophosphate
μX-ray absorption spectroscopy
Zn environment

UNIGE affiliation entities

Not a UNIGE publication

UNIGE research groups

Mécanismes physiopathologiques de l'arthrose (960)

Citation (ISO format)

DESSOMBZ, Arnaud et al. Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn²⁺cations in cartilage and meniscus calcifications. In: Journal of trace elements in medicine and biology, 2013, vol. 27, n° 4, p. 326–333. doi: 10.1016/j.jtemb.2013.02.001

Article (Published version)

Identifiers

PID : unige:80283
DOI : 10.1016/j.jtemb.2013.02.001
PMID : 23582484

Additional URL for this publication

https://www.sciencedirect.com/science/article/pii/S0946672X13000084

Journal ISSN

0946-672X

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02/02/2016 16:31:00

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02/02/2016 16:31:00

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30/04/2026 12:02:52

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30/04/2026 12:02:52

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30/04/2026 12:04:42

Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn²⁺cations in cartilage and meniscus calcifications

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Abstract

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Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn2+cations in cartilage and meniscus calcifications

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation

Combining μX-ray fluorescence, μXANES and μXRD to shed light on Zn²⁺cations in cartilage and meniscus calcifications