Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Emre, Yalin; Jemelin, Stéphane; Imhof, Beat

doi:10.3791/53314

Scientific article

English

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Contributors

Emre, Yalin; Jemelin, Stéphane; Imhof, Beat

Published in

Journal of visualized experiments, no. 105

Publication date

2015

Abstract

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in vivo is crucial for understanding the molecular basis of leukocyte transendothelial migration and interaction with vascular endothelium. One powerful approach involves intravital microscopy on transgenic mice expressing fluorescent proteins in cells of interest. Here we present a protocol for imaging monocytes and neutrophils in the CX3CR1(gfp/wt) mouse i.v. injected with orange dye-labeled neutrophils with an inverted confocal microscope. Time-lapse movies gathered from 30 min to several hours of imaging allow the analysis of leukocyte behavior in mesenteric veins under both steady state and inflammatory conditions. We also describe the steps to locally induce blood vessel inflammation with TLR2/TLR1 agonist Pam3SK4 and monitor the subsequent recruitment of neutrophils and monocytes. The presented technique can also be used to monitor other populations of leukocytes and investigate molecules implicated in leukocyte recruitment or trafficking using other stimuli or transgenic mice.

UNIGE affiliation entities

Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

UNIGE research groups

Molécules d'adhésion et processus de migration cellulaire (167)

Citation (ISO format)

EMRE, Yalin, JEMELIN, Stéphane, IMHOF, Beat. Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time. In: Journal of visualized experiments, 2015, n° 105. doi: 10.3791/53314

Article (Published version)

Identifiers

PID : unige:79991
DOI : 10.3791/53314
PMID : 26649781

Journal ISSN

1940-087X

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Creation

25/01/2016 08:02:00

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25/01/2016 08:02:00

Update

15/03/2023 00:05:58

Status update

15/03/2023 00:05:58

Last indexation

31/10/2024 02:37:32

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation