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Establishing Corneal Cross-Linking With Riboflavin and UV-A in the Mouse Cornea In Vivo: Biomechanical Analysis

Randleman, J. Bradley
Published in Investigative ophthalmology & visual science. 2015, vol. 56, no. 11, 6581
Abstract Purpose: To establish corneal cross-linking (CXL) with riboflavin and UV-A in in the mouse cornea in vivo and to develop tools to measure the biomechanical changes observed. Methods: A total of 55 male C57BL/6 wild-type mice (aged 5 weeks) were divided into 14 groups. Standard CXL parameters were adapted to the anatomy of the mouse cornea, and riboflavin concentration (0.1%–0.5%) and fluence series (0.09–5.4 J/cm2) were performed on the assumption of the endothelial damage thresholds. Untreated and riboflavin only corneas were used as controls. Animals were killed at 30 minutes and at 1 month after CXL. Corneas were harvested. Two-dimensional (2D) biomechanical testing was performed using a customized corneal holder in a commercially available stress-strain extensometer/indenter. Both elastic and viscoelastic analyses were performed. Statistical inference was performed using t-tests and specific mathematical models fitted to the experimental stress-strain and stress-relaxation data. Adjusted P values by the method of Benjamini and Hochberg are reported. Results: For all CXL treatment groups, stress-relaxation showed significant differences (P < 0.0001) after 120 seconds of constant strain application, with cross-linked corneas maintaining a higher stress (441 ± 40 kPa) when compared with controls (337 ± 39 kPa). Stress-strain analysis confirmed these findings but was less sensitive to CXL-induced changes: at 0.5% of strain, cross-linked corneas remained at higher stress (778 ± 111 kPa) when compared with controls (659 ± 121 kPa). Conclusions: Cross-linking was induced in the mouse cornea in vivo, and its biomechanical effect successfully measured. This could create opportunities to study molecular pathways of CXL in transgenic mice.
Keywords AnimalsBiomechanical PhenomenaCornea/drug effects/physiopathology/radiation effectsCross-Linking Reagents/pharmacologyDisease ModelsAnimalKeratoconus/drug therapy/physiopathologyMaleMiceMiceInbred C57BLPhotosensitizing Agents/pharmacologyRiboflavin/pharmacologyUltraviolet Rays
PMID: 26465887
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HAMMER, Arthur et al. Establishing Corneal Cross-Linking With Riboflavin and UV-A in the Mouse Cornea In Vivo: Biomechanical Analysis. In: Investigative ophthalmology & visual science, 2015, vol. 56, n° 11, p. 6581. doi: 10.1167/iovs.15-17426 https://archive-ouverte.unige.ch/unige:79867

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Deposited on : 2016-01-22

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