The determination of 5'-ends of RNA molecules is important for understanding various steps of gene expression and regulation in all organisms, such as transcription initiation, RNA maturation, and degradation. While previous methods like Phosphorylation Assay By Ligation of Oligonucleotides, Rapid Amplification of cDNA Ends, Capped Analysis of Gene expression, tag RNA-seq and differential RNA-seq have their own specifications and limitations, Exact Mapping Of Transcriptome Ends (EMOTE) assay has been designed to determine the 5'-ends of RNAs on a transcriptome-wide scale. EMOTE-conv exploits the raw sequence reads generated from the EMOTE assay which is, to the best of our knowledge, the only method that can map the exact RNA 5'-ends of of all types on a transcriptome wide scale. It converts EMOTE data into the quantified list of genomic positions that corresponds to the 5'-end of RNA, signifying 5'-base RNA and the other related genomic information. EMOTE-conv is platform-independent, user-friendly and easy-to-use. It can be used with the data generated from other sequencing platforms with a converter as well as the data generated from any organism, species or strains. The EMOTE-conv software is available at: http://sourceforge.net/projects/emoteconv