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Title

ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome biogenesis

Authors
Kindbeiter, Karine
Belin, Stéphane
Duret, Laurent
Bezin, Laurent
Diaz, Jean-Jacques
Published in Molecular and Cellular Proteomics. 2008, vol. 7, no. 3, p. 546-559
Abstract Proteomics analyses of human nucleoli provided molecular bases for an understanding of the multiple functions fulfilled by these nuclear domains. However, the biological roles of about 100 of the identified proteins are unpredictable. The present study describes the functional characterization of one of these proteins, ISG20L2. We demonstrate that ISG20L2 is a 3' to 5' exoribonuclease involved in ribosome biogenesis at the level of 5.8 S rRNA maturation, more specifically in the processing of the 12 S precursor rRNA. The use of truncated forms of ISG20L2 demonstrated that its N-terminal half promotes the nucleolar localization and suggested that its C-terminal half bears the exoribonuclease activity. Identification of the binding partners of ISG20L2 confirmed its involvement in the biogenesis of the large ribosomal subunit. These results strongly support the notion that, in human, as it was demonstrated in yeast, 5.8 S rRNA maturation requires several proteins in addition to the exosome complex. Furthermore this observation greatly sustains the idea that the extremely conserved need for correctly processed rRNAs in vertebrates and yeast is achieved by close but different mechanisms.
Keywords Amino Acid SequenceAnimalsCell Nucleolus/enzymologyComputational BiologyExodeoxyribonucleases/chemistry/metabolismGene SilencingHela CellsHumansImmunoprecipitationMolecular Sequence DataMutant Proteins/metabolismPhylogenyProtein BindingProtein Structure TertiaryProtein TransportRNA Precursors/metabolismRNA Ribosomal/metabolismRecombinant Fusion Proteins/metabolismRibosomal Proteins/metabolismRibosomes/metabolismSaccharomyces cerevisiae Proteins/geneticsSubstrate SpecificityVertebrates
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PMID: 18065403
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Other version: http://www.mcponline.org/cgi/content/full/7/3/546
Structures
Research group Groupe de Protéomique biomédicale (635)
Citation
(ISO format)
COUTE, Yohann et al. ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome biogenesis. In: Molecular and Cellular Proteomics, 2008, vol. 7, n° 3, p. 546-559. https://archive-ouverte.unige.ch/unige:795

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Deposited on : 2009-02-13

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