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Title

Decay-Initiating Endoribonucleolytic Cleavage by RNase Y Is Kept under Tight Control via Sequence Preference and Sub-cellular Localisation
Authors
Khemici, Vanessa
Prados, Julien
Linder, Patrick
Redder, Peter
Published in PLOS genetics. 2015, vol. 11, no. 10, e1005577
Abstract Bacteria depend on efficient RNA turnover, both during homeostasis and when rapidly altering gene expression in response to changes. Nevertheless, remarkably few details are known about the rate-limiting steps in targeting and decay of RNA. The membrane-anchored endoribonuclease RNase Y is a virulence factor in Gram-positive pathogens. We have obtained a global picture of Staphylococcus aureus RNase Y sequence specificity using RNA-seq and the novel transcriptome-wide EMOTE method. Ninety-nine endoribonucleolytic sites produced in vivo were precisely mapped, notably inside six out of seven genes whose half-lives increase the most in an RNase Y deletion mutant, and additionally in three separate transcripts encoding degradation ribonucleases, including RNase Y itself, suggesting a regulatory network. We show that RNase Y is required to initiate the major degradation pathway of about a hundred transcripts that are inaccessible to other ribonucleases, but is prevented from promiscuous activity by membrane confinement and sequence preference for guanosines.
Identifiers
PMID: 26473962
Full text
Article (Published version) (2.6 MB) - public document Free access
Structures
Faculté de médecine / Section de médecine clinique / Département de psychiatrie
Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group Acquisition et expression de facteurs de virulence chez Staphylococcus aureus (86)
Citation
(ISO format) 		KHEMICI, Vanessa et al. Decay-Initiating Endoribonucleolytic Cleavage by RNase Y Is Kept under Tight Control via Sequence Preference and Sub-cellular Localisation. In: PLOS genetics, 2015, vol. 11, n° 10, p. e1005577. doi: 10.1371/journal.pgen.1005577 https://archive-ouverte.unige.ch/unige:79361

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Deposited on : 2016-01-14

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