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Toward p-Octiphenyl ß-Barrel RNases

Published in Macromolecules. 2002, vol. 35, no. 5, p. 1549-1555
Abstract A synthetic barrel-stave supramolecule with p-oligophenyl “staves” and ß-sheet “hoops” is shown to catalyze the hydrolysis of a doubly labeled homooligomeric model RNA 32 000 times compared to imidazole in water at nanomolar concentrations, 50 °C, and pH 7.0. RNase activity of this rigid-rod ß-barrel is shown to depend on pH (maximal at pH 5.5) and Zn2+ (up to 7-fold increase). Denaturation kinetics suggest that an intact suprastructure is essential for activity and that RNA and Zn2+ exhibit a stabilizing template effect. Product analysis by HPLC is indicative for selective RNase activity with respect to substrate length.
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BAUMEISTER, Bodo, MATILE, Stefan. Toward p-Octiphenyl ß-Barrel RNases. In: Macromolecules, 2002, vol. 35, n° 5, p. 1549-1555. doi: 10.1021/ma011612m https://archive-ouverte.unige.ch/unige:7928

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Deposited on : 2010-06-21

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