Scientific article
Open access

Epigenetic Alterations at Genomic Loci Modified by Gene Targeting in Arabidopsis thaliana

Published inPloS one, vol. 8, no. 12, e85383
Publication date2013

Gene Targeting (GT) is the integration of an introduced vector into a specific chromosomal site, via homologous recombination. It is considered an effective tool for precise genome editing, with far-reaching implications in biological research and biotechnology, and is widely used in mice, with the potential of becoming routine in many species. Nevertheless, the epigenetic status of the targeted allele remains largely unexplored. Using GT-modified lines of the model plant Arabidopsis thaliana, we show that the DNA methylation profile of the targeted locus is changed following GT. This effect is non-directional as methylation can be either completely lost, maintained with minor alterations or show instability in the generations subsequent to GT. As DNA methylation is known to be involved in several cellular processes, GT-related alterations may result in unexpected or even unnoticed perturbations. Our analysis shows that GT may be used as a new tool for generating epialleles, for example, to study the role of gene body methylation. In addition, the analysis of DNA methylation at the targeted locus may be utilized to investigate the mechanism of GT, many aspects of which are still unknown.

Citation (ISO format)
LIEBERMAN LAZAROVICH, Michal et al. Epigenetic Alterations at Genomic Loci Modified by Gene Targeting in Arabidopsis thaliana. In: PloS one, 2013, vol. 8, n° 12, p. e85383. doi: 10.1371/journal.pone.0085383
Main files (1)
Article (Published version)
ISSN of the journal1932-6203

Technical informations

Creation01/05/2016 12:00:00 PM
First validation01/05/2016 12:00:00 PM
Update time03/15/2023 12:01:56 AM
Status update03/15/2023 12:01:56 AM
Last indexation02/12/2024 11:23:43 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack