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Scientific article
English

Direct Observation of Anion-Mediated Translocation of Fluorescent Oligoarginine Carriers into and across Bulk Liquid and Anionic Bilayer Membranes

Published inChemBioChem, vol. 6, no. 1, no. Special Issue : EMBO Symposium: Chemistry Meets Biology, p. 114-122
Publication date2005
Abstract

The recent hypothesis that counteranion-mediated dynamic inversion of charge and solubility might contribute to diverse functions of oligoarginines in biomembranes was tested with two fluorescently labelled oligomers, FL-R8, one of the most active cell-penetrating peptides, and its longer version, FL-R16. We report evidence for counteranion-mediated phase transfer from water into bulk chloroform and anionic lipid-bilayer membranes as well as reverse-phase transfer from bulk chloroform and across intact lipid-bilayer membranes into water. The differences found between FL-R8 and FL-R16 with regard to location in the bilayer and reverse-phase transfer from bulk and lipid-bilayer membranes into water implied that the reported results may be relevant for biological function.

Keywords
  • Anions
  • Bioorganic chemistry
  • Ion pairs
  • Lipid bilayers
  • Oligoarginines
Citation (ISO format)
SAKAI, Naomi et al. Direct Observation of Anion-Mediated Translocation of Fluorescent Oligoarginine Carriers into and across Bulk Liquid and Anionic Bilayer Membranes. In: ChemBioChem, 2005, vol. 6, n° 1, p. 114–122. doi: 10.1002/cbic.200400256
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ISSN of the journal1439-4227
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