en
Scientific article
English

Microbiota depletion promotes browning of white adipose tissue and reduces obesity

Published inNature medicine, vol. 21, no. 12, p. 1497-1503
Publication date2015
Abstract

Brown adipose tissue (BAT) promotes a lean and healthy phenotype and improves insulin sensitivity1. In response to cold or exercise, brown fat cells also emerge in the white adipose tissue (WAT; also known as beige cells), a process known as browning2–4. Here we show that the development of functional beige fat in the inguinal subcutaneous adipose tissue (ingSAT) and perigonadal visceral adipose tissue (pgVAT) is promoted by the depletion of microbiota either by means of antibiotic treatment or in germ-free mice. This leads to improved glucose tolerance and insulin sensitivity and decreased white fat and adipocyte size in lean mice, obese leptin-deficient (ob/ob) mice and high-fat diet (HFD)-fed mice. Such metabolic improvements are mediated by eosinophil infiltration, enhanced type 2 cytokine signaling and M2 macrophage polarization in the subcutaneous white fat depots of microbiota-depleted animals. The metabolic phenotype and the browning of the subcutaneous fat are impaired by the suppression of type 2 cytokine signaling, and they are reversed by recolonization of the antibiotic-treated or germfree mice with microbes. These results provide insight into the microbiota-fat signaling axis and beige-fat development in health and metabolic disease.

Keywords
  • Adipocytes/cytology/drug effects/metabolism
  • Adipose Tissue, Brown/drug effects/metabolism
  • Adipose Tissue, White/drug effects/metabolism
  • Animals
  • Cell Size/drug effects
  • Cytokines/metabolism
  • Gene Expression Regulation/drug effects
  • Germ-Free Life
  • Glucose/metabolism
  • Glucose Tolerance Test
  • Insulin/pharmacology
  • Intra-Abdominal Fat/drug effects/metabolism
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Microbiota/drug effects
  • Obesity/microbiology/pathology
  • RNA, Messenger/genetics/metabolism
  • Signal Transduction/drug effects
  • Subcutaneous Fat/drug effects/metabolism
Citation (ISO format)
SUAREZ ZAMORANO, Nicolas et al. Microbiota depletion promotes browning of white adipose tissue and reduces obesity. In: Nature medicine, 2015, vol. 21, n° 12, p. 1497–1503. doi: 10.1038/nm.3994
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1078-8956
645views
12downloads

Technical informations

Creation12/07/2015 4:46:00 PM
First validation12/07/2015 4:46:00 PM
Update time03/15/2023 12:01:00 AM
Status update03/15/2023 12:01:00 AM
Last indexation01/16/2024 7:55:32 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack