UNIGE document Scientific Article
previous document  unige:78847  next document
add to browser collection
Title

Using digital RNA counting and flow cytometry to compare mRNA with protein expression in acute leukemias

Authors
Fernandez, Paula
Solenthaler, Max
Spertini, Olivier
Quarroz, Stephane
Rovo, Alicia
Lovey, Pierre-Yves
Leoncini, Leda
Ruault-Jungblut, Sylvie
show hidden authors show all authors [1 - 13]
Published in PLOS ONE. 2012, vol. 7, no. 11, p. e49010
Abstract The diagnosis of malignant hematologic diseases has become increasingly complex during the last decade. It is based on the interpretation of results from different laboratory analyses, which range from microscopy to gene expression profiling. Recently, a method for the analysis of RNA phenotypes has been developed, the nCounter technology (Nanostring® Technologies), which allows for simultaneous quantification of hundreds of RNA molecules in biological samples. We evaluated this technique in a Swiss multi-center study on eighty-six samples from acute leukemia patients.
Keywords Antigens, CD/genetics/metabolismBlood Cells/metabolismBone Marrow Cells/metabolismFlow Cytometry/methodsGenomics/methodsHumansImmunophenotypingLeukemia/diagnosis/genetics/metabolismProteomeProteomics/methodsTranscriptome
Identifiers
PMID: 23152841
Full text
Article (Published version) (681 Kb) - public document Free access
Structures
Research group Analyses cellulaires et moléculaires des hémopathies malignes (929)
Projects FNS: 320030_132926
Fondation Dr Dubois Ferrière / Dinu Lipatti
Citation
(ISO format)
FERNANDEZ, Paula et al. Using digital RNA counting and flow cytometry to compare mRNA with protein expression in acute leukemias. In: PLOS ONE, 2012, vol. 7, n° 11, p. e49010. https://archive-ouverte.unige.ch/unige:78847

50 hits

26 downloads

Update

Deposited on : 2015-12-21

Export document
Format :
Citation style :