UNIGE document Scientific Article
previous document  unige:7871  next document
add to browser collection

Enzyme screening with synthetic multifunctional pores: Focus on biopolymers

Published in Proceedings of the National Academy of Sciences. 2003, vol. 100, no. 21, p. 11964-11969
Abstract This report demonstrates that a single set of identical synthetic multifunctional pores can detect the activity of many different enzymes. Enzymes catalyzing either synthesis or degradation of DNA (exonuclease III or polymerase I), RNA (RNase A), polysaccharides (heparinase I, hyaluronidase, and galactosyltransferase), and proteins (papain, ficin, elastase, subtilisin, and pronase) are selected to exemplify this key characteristic of synthetic multifunctional pore sensors. Because anionic, cationic, and neutral substrates can gain access to the interior of complementarily functionalized pores, such pores can be the basis for very user-friendly screening of a broad range of enzymes.
PMID: 14530413
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
SORDE, Nathalie, DAS, Gopal, MATILE, Stefan. Enzyme screening with synthetic multifunctional pores: Focus on biopolymers. In: Proceedings of the National Academy of Sciences, 2003, vol. 100, n° 21, p. 11964-11969. doi: 10.1073/pnas.2132894100 https://archive-ouverte.unige.ch/unige:7871

396 hits



Deposited on : 2010-06-21

Export document
Format :
Citation style :