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High-allelic variability in HLA-C mRNA expression: association with HLA-extended haplotypes

Bettens, F
Brunet, L
Published in Genes and Immunity. 2014, vol. 15, no. 3, p. 176-181
Abstract Human leukocyte antigen (HLA)-C is a clinically relevant transplantation antigen in unrelated hematopoietic stem cell and cord blood transplantation. Furthermore, HLA-C antigens, as ligands for killer immunoglobulin-like receptors expressed on natural killer cells, have a central role in HIV control. Several studies have reported significant correlations between HLA-C mRNA and cell surface expression with polymorphisms in the 5'- and 3'-regions of the HLA-C locus. We determined HLA-C mRNA in blood donors by using locus as well as allele-specific real-time-PCR and focused the analysis on HLA-extended haplotypes. High inter-individual variability of mRNA expression was disclosed. A lower inter-individual variability for C*07:01 but a higher variability for C*06:02, C*04:01 and C*03:04 alleles were detected. The previously reported associations between HLA-C cell surface expression and -32 kb/-35 kb single nucleotide polymorphisms were not confirmed. Related and unrelated individuals sharing the same two A-B-C-DRB1 or B-C haplotypes show strikingly similar levels of HLA-C mRNA expression in each of the different haplotypic combinations tested. Altogether, our results suggest that HLA-C expression levels best correlate with the extended HLA haplotype rather than with the allotype or with polymorphisms in the 5'-region of the HLA-C locus.
Keywords AllelesGene ExpressionHLA-C Antigens/geneticsHaplotypesHumansPolymorphism, Single NucleotideRNA, Messenger
PMID: 24500399
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Research group Compatibilité tissulaire et transplantation clinique (562)
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BETTENS, F, BRUNET, L, TIERCY, Jean-Marie. High-allelic variability in HLA-C mRNA expression: association with HLA-extended haplotypes. In: Genes and Immunity, 2014, vol. 15, n° 3, p. 176-181. doi: 10.1038/gene.2014.1 https://archive-ouverte.unige.ch/unige:78614

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Deposited on : 2015-12-14

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