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Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion

Meissner, Markus
Schlüter, Dirk
Published in Science. 2002, vol. 298, no. 5594, p. 837-840
Abstract Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.
Keywords AnimalsCalcimycin/pharmacologyCalcium/metabolismCell LineCells, CulturedExocytosisGenetic VectorsHumansMiceMovementNonmuscle Myosin Type IIA/genetics/physiologyOrganelles/metabolismProtozoan Proteins/genetics/physiologyTetracycline/pharmacologyToxoplasma/genetics/growth & development/pathogenicity/physiologyToxoplasmosis, Animal/parasitologyTrans-Activators/metabolismTransfectionTransgenesVirulenceVirulence Factors/physiology
PMID: 12399593
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Research group Biologie d'un parasite intracellulaire obligatoire (773)
(ISO format)
MEISSNER, Markus, SCHLÜTER, Dirk, SOLDATI-FAVRE, Dominique. Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion. In: Science, 2002, vol. 298, n° 5594, p. 837-840. https://archive-ouverte.unige.ch/unige:78026

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Deposited on : 2015-12-02

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