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Scientific article
Open access
English

Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion

Published inScience, vol. 298, no. 5594, p. 837-840
Publication date2002
Abstract

Obligate intracellular apicomplexan parasites rely on gliding motion powered by their actomyosin system to disperse throughout tissues and to penetrate host cells. Toxoplasma gondii myosin A has been implicated in this process, but direct proof has been lacking. We designed a genetic screen to generate a tetracycline-inducible transactivator system in T. gondii. The MyoA gene was disrupted in the presence of a second regulatable copy of MyoA. Conditional removal of this myosin caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.

Keywords
  • Animals
  • Calcimycin/pharmacology
  • Calcium/metabolism
  • Cell Line
  • Cells, Cultured
  • Exocytosis
  • Genetic Vectors
  • Humans
  • Mice
  • Movement
  • Nonmuscle Myosin Type IIA/genetics/physiology
  • Organelles/metabolism
  • Protozoan Proteins/genetics/physiology
  • Tetracycline/pharmacology
  • Toxoplasma/genetics/growth & development/pathogenicity/physiology
  • Toxoplasmosis, Animal/parasitology
  • Trans-Activators/metabolism
  • Transfection
  • Transgenes
  • Virulence
  • Virulence Factors/physiology
Citation (ISO format)
MEISSNER, Markus, SCHLÜTER, Dirk, SOLDATI-FAVRE, Dominique. Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion. In: Science, 2002, vol. 298, n° 5594, p. 837–840. doi: 10.1126/science.1074553
Main files (1)
Article (Accepted version)
accessLevelPublic
Identifiers
ISSN of the journal0036-8075
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522downloads

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