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Title

Functional and biophysical analyses of the class XIV Toxoplasma gondii myosin D.

Authors
Herm-Götz, Angelika
Delbac, Frêdêric
Weiss, Stefan
Nyitrai, Miklos
Stratmann, Rolf
Tomavo, Stanislas
Sibley, L David
Geeves, Michael A
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Published in Journal of Muscle Research and Cell Motility. 2006, vol. 27, no. 2, p. 139-151
Abstract The obligate intracellular parasite Toxoplasma gondii uses gliding motility to migrate across the biological barriers of the host and to invade cells. This unique form of locomotion requires an intact actin cytoskeleton and involves at least one motor protein (TgMyoA) that belongs to the class XIV of the myosin superfamily. TgMyoA is anchored in the inner membrane complex and is essential for the gliding motion, host cell invasion and egress of T. gondii tachyzoites. TgMyoD is the smallest T. gondii myosin and is structurally very closely related to TgMyoA. We show here that TgMyoD exhibits similar transient kinetic properties as the fast single-headed TgMyoA. To determine if TgMyoD also contributes to parasite gliding motility, the TgMyoD gene was disrupted by double homologous recombination. In contrast to TgMyoA, TgMyoD gene is dispensable for tachyzoite propagation and motility. Parasites lacking TgMyoD glide normally and their virulence is not compromised in mice. The fact that TgMyoD is predominantly expressed in bradyzoites explains the absence of a phenotype observed with myodko in tachyzoites and does not exclude a role of this motor in gliding that would be restricted to the cyst forming but nevertheless motile stage of the parasite.
Keywords AnimalsCell Movement/geneticsGene DeletionMyoD Protein/genetics/metabolismProtozoan Proteins/genetics/metabolismToxoplasma/genetics/metabolism
Identifiers
PMID: 16470333
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Article (Author postprint) (674 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Biologie d'un parasite intracellulaire obligatoire (773)
Project FNS: 310030_147118
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HERM-GÖTZ, Angelika et al. Functional and biophysical analyses of the class XIV Toxoplasma gondii myosin D. In: Journal of Muscle Research and Cell Motility, 2006, vol. 27, n° 2, p. 139-151. https://archive-ouverte.unige.ch/unige:78025

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Deposited on : 2015-12-02

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