UNIGE document Scientific Article
previous document  unige:7780  next document
add to browser collection
Title

G-Quartet Self-Assembly under Osmotic Pressure: Remote Control by Vesicle Shrinking Rather Than Stress

Authors
Published in Chirality. 2003, vol. 15, no. 9, p. 766-771
Abstract Does osmotic pressure stimulate assembly or disassembly of supramolecules in vesicles? Self-assembly was conceivable as intravesicular response to osmotic shrinking upon application of extravesicular overpressure, whereas disassembly was conceivable as a response to bilayer stress in hyperosmotic vesicles. Self-assembly of guanosine 5'-monophosphates (GMPs) into G-quartets was selected to investigate the nature of remote control of supramolecular chemistry within vesicles by osmotic pressure. Using circular dichroism spectroscopy to selectively detect G-quartets, we found that extravesicular overpressure stimulates intravesicular self-assembly, whereas underpressure stimulates disassembly. G-quartet self-assembly by osmotic pressure exhibited ion-selective metal-cation templation, as expected. The key conclusions are that supramolecular chemistry within vesicles is governed by vesicle shape rather than vesicle stress and that detection of osmotic pressure by CD spectroscopy is an interesting alternative to the commonly used methods based on fluorescence self-quenching.
Keywords Circular dichroismG-quartetOsmotic pressureSelf-assemblySupramolecular chemistryVesicles
Identifiers
PMID: 14556212
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
SAKAI, Naomi, MATILE, Stefan. G-Quartet Self-Assembly under Osmotic Pressure: Remote Control by Vesicle Shrinking Rather Than Stress. In: Chirality, 2003, vol. 15, n° 9, p. 766-771. https://archive-ouverte.unige.ch/unige:7780

221 hits

0 download

Update

Deposited on : 2010-06-21

Export document
Format :
Citation style :