UNIGE document Scientific Article
previous document  unige:77544  next document
add to browser collection

Treatment with CB2 agonist JWH-133 reduces histological features associated with erectile dysfunction in hypercholesterolemic mice

Fraga-Silva, Rodrigo Araujo
Costa-Fraga, Fabiana Pereira
Faye, Younouss
Savergnini, Silvia Quintao
show hidden authors show all authors [1 - 11]
Published in Clinical & developmental immunology. 2013, vol. 2013, 263846
Abstract Hypercholesterolemia is one of the most important risk factors for erectile dysfunction, mostly due to the impairment of oxidative stress and endothelial function in the penis. The cannabinoid system might regulate peripheral mechanisms of sexual function; however, its role is still poorly understood. We investigated the effects of CB2 activation on oxidative stress and fibrosis within the corpus cavernosum of hypercholesterolemic mice. Apolipoprotein-E-knockout mice were fed with a western-type diet for 11 weeks and treated with JWH-133 (selective CB2 agonist) or vehicle during the last 3 weeks. CB2 receptor expression, total collagen content, and reactive oxygen species (ROS) production within the penis were assessed. In vitro corpus cavernosum strips preparation was performed to evaluate the nitric oxide (NO) bioavailability. CB2 protein expression was shown in cavernosal endothelial and smooth muscle cells of wild type and hypercholesterolemic mice. Treatment with JWH-133 reduced ROS production and NADPH-oxidase expression in hypercholesterolemic mice penis. Furthermore, JWH-133 increased endothelial NO synthase expression in the corpus cavernosum and augmented NO bioavailability. The decrease in oxidative stress levels was accompanied with a reduction in corpus cavernosum collagen content. In summary, CB2 activation decreased histological features, which were associated with erectile dysfunction in hypercholesterolemic mice.
Keywords AnimalsCannabinoids/administration & dosage/pharmacologyDisease Models, AnimalErectile Dysfunction/complications/drug therapy/metabolismFibrosisHypercholesterolemia/complications/metabolismLipids/bloodMaleMiceMice, KnockoutNADPH Oxidase/metabolismNitric Oxide/metabolismNitric Oxide Synthase Type III/metabolismPenis/drug effects/metabolism/pathologyReactive Oxygen Species/metabolismReceptor, Cannabinoid, CB2/agonists/metabolism
PMID: 24302957
Full text
Article (Published version) (2.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research group L'athérosclérose et ses complications cliniques (591)
(ISO format)
FRAGA-SILVA, Rodrigo Araujo et al. Treatment with CB2 agonist JWH-133 reduces histological features associated with erectile dysfunction in hypercholesterolemic mice. In: Clinical & developmental immunology, 2013, vol. 2013, p. 263846. doi: 10.1155/2013/263846 https://archive-ouverte.unige.ch/unige:77544

381 hits

0 download


Deposited on : 2015-11-23

Export document
Format :
Citation style :