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Title

Cross-presenting CD103+ dendritic cells are protected from influenza virus infection

Authors
Helft, Julie
Manicassamy, Balaji
Guermonprez, Pierre
Hashimoto, Daigo
Silvin, Aymeric
Agudo, Judith
Brown, Brian D
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Published in Journal of Clinical Investigation. 2012, vol. 122, no. 11, p. 4037-4047
Abstract CD8+ cytotoxic T cells are critical for viral clearance from the lungs upon influenza virus infection. The contribution of antigen cross-presentation by DCs to the induction of anti-viral cytotoxic T cells remains controversial. Here, we used a recombinant influenza virus expressing a nonstructural 1-GFP (NS1-GFP) reporter gene to visualize the route of antigen presentation by lung DCs upon viral infection in mice. We found that lung CD103+ DCs were the only subset of cells that carried intact GFP protein to the draining LNs. Strikingly, lung migratory CD103+ DCs were not productively infected by influenza virus and thus were able to induce virus-specific CD8+ T cells through the cross-presentation of antigens from virally infected cells. We also observed that CD103+ DC resistance to infection correlates with an increased anti-viral state in these cells that is dependent on the expression of type I IFN receptor. These results show that efficient cross-priming by migratory lung DCs is coupled to the acquisition of an anti-viral status, which is dependent on the type I IFN signaling pathway.
Keywords AnimalsAntigen PresentationAntigens, CDAntigens, Viral/genetics/immunologyCD8-Positive T-Lymphocytes/immunology/pathologyCell LineDendritic Cells/immunology/pathologyDogsGreen Fluorescent Proteins/genetics/immunologyInfluenza A virus/genetics/immunologyIntegrin alpha ChainsInterferon Type I/genetics/immunologyLung/immunology/pathology/virologyMiceOrthomyxoviridae Infections/genetics/immunology/prevention & controlReceptor, Interferon alpha-beta/genetics/immunologyRecombinant Fusion Proteins/genetics/immunologySignal Transduction/genetics/immunologyViral Nonstructural Proteins/genetics/immunology
Identifiers
PMID: 23041628
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HELFT, Julie et al. Cross-presenting CD103+ dendritic cells are protected from influenza virus infection. In: Journal of Clinical Investigation, 2012, vol. 122, n° 11, p. 4037-4047. https://archive-ouverte.unige.ch/unige:77415

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Deposited on : 2015-11-18

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