DNA-Methylation Patterns in Trisomy 21 Using Cells from Monozygotic Twins

Sailani, Mohammad Reza; Santoni, Federico; Letourneau, Audrey; Borel, Christelle; Makrythanasis, Periklis; Hibaoui, Youssef; Popadin, Konstantin; Bonilla Bustillo, Ximena; Guipponi, Michel; Gehrig, Corinne; Vannier, Anne; Carre-Pigeon, Frederique; Feki, Anis; Nizetic, Dean; Antonarakis, Stylianos

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Title		DNA-Methylation Patterns in Trisomy 21 Using Cells from Monozygotic Twins
Authors		Sailani, Mohammad Reza Santoni, Federico Letourneau, Audrey Borel, Christelle Makrythanasis, Periklis Hibaoui, Youssef Popadin, Konstantin Bonilla Bustillo, Ximena Guipponi, Michel Gehrig, Corinne Vannier, Anne Carre-Pigeon, Frederique Feki, Anis Nizetic, Dean Antonarakis, Stylianos show all authors [1 - 15]
Published in		PLOS ONE. 2015, vol. 10, no. 8, p. e0135555
Abstract		DNA methylation is essential in mammalian development. We have hypothesized that methylation differences induced by trisomy 21 (T21) contribute to the phenotypic characteristics and heterogeneity in Down syndrome (DS). In order to determine the methylation differences in T21 without interference of the interindividual genomic variation, we have used fetal skin fibroblasts from monozygotic (MZ) twins discordant for T21. We also used skin fibroblasts from MZ twins concordant for T21, normal MZ twins without T21, and unrelated normal and T21 individuals. Reduced Representation Bisulfite Sequencing (RRBS) revealed 35 differentially methylated promoter regions (DMRs) (Absolute methylation differences = 25%, FDR < 0.001) in MZ twins discordant for T21 that have also been observed in comparison between unrelated normal and T21 individuals. The identified DMRs are enriched for genes involved in embryonic organ morphogenesis (FDR = 1.60 e -03) and include genes of the HOXB and HOXD clusters. These DMRs are maintained in iPS cells generated from this twin pair and are correlated with the gene expression changes. We have also observed an increase in DNA methylation level in the T21 methylome compared to the normal euploid methylome. This observation is concordant with the up regulation of DNA methyltransferase enzymes (DNMT3B and DNMT3L) and down regulation of DNA demethylation enzymes (TET2 and TET3) observed in the iPSC of the T21 versus normal twin. Altogether, the results of this study highlight the epigenetic effects of the extra chromosome 21 in T21 on loci outside of this chromosome that are relevant to DS associated phenotypes.
Identifiers		DOI: 10.1371/journal.pone.0135555 PMID: 26317209
Full text		Article (Published version) (2 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement
Research group		Pathologie Moléculaire de la Trisomie 21 et le Génome Humain (248)
Citation (ISO format)		SAILANI, Mohammad Reza et al. DNA-Methylation Patterns in Trisomy 21 Using Cells from Monozygotic Twins. In: PLOS ONE, 2015, vol. 10, n° 8, p. e0135555. https://archive-ouverte.unige.ch/unige:77402