UNIGE document Scientific Article
previous document  unige:77339  next document
add to browser collection
Title

Fundamental Roles of the Golgi-Associated Toxoplasma Aspartyl Protease, ASP5, at the Host-Parasite Interface

Authors
Di Cristina, Manlio
Romano, Julia
show hidden authors show all authors [1 - 16]
Published in PLOS Pathogens. 2015, vol. 11, no. 10, p. e1005211
Abstract Toxoplasma gondii possesses sets of dense granule proteins (GRAs) that either assemble at, or cross the parasitophorous vacuole membrane (PVM) and exhibit motifs resembling the HT/PEXEL previously identified in a repertoire of exported Plasmodium proteins. Within Plasmodium spp., cleavage of the HT/PEXEL motif by the endoplasmic reticulum-resident protease Plasmepsin V precedes trafficking to and export across the PVM of proteins involved in pathogenicity and host cell remodelling. Here, we have functionally characterized the T. gondii aspartyl protease 5 (ASP5), a Golgi-resident protease that is phylogenetically related to Plasmepsin V. We show that deletion of ASP5 causes a significant loss in parasite fitness in vitro and an altered virulence in vivo. Furthermore, we reveal that ASP5 is necessary for the cleavage of GRA16, GRA19 and GRA20 at the PEXEL-like motif. In the absence of ASP5, the intravacuolar nanotubular network disappears and several GRAs fail to localize to the PVM, while GRA16 and GRA24, both known to be targeted to the host cell nucleus, are retained within the vacuolar space. Additionally, hypermigration of dendritic cells and bradyzoite cyst wall formation are impaired, critically impacting on parasite dissemination and persistence. Overall, the absence of ASP5 dramatically compromises the parasite's ability to modulate host signalling pathways and immune responses.
Identifiers
PMID: 26473595
Full text
Article (Author postprint) (14.1 MB) - public document Free access
Structures
Research group Biologie d'un parasite intracellulaire obligatoire (773)
Project FNS: FN3100A0-116722
Citation
(ISO format)
HAMMOUDI, Pierre-Mehdi et al. Fundamental Roles of the Golgi-Associated Toxoplasma Aspartyl Protease, ASP5, at the Host-Parasite Interface. In: PLOS Pathogens, 2015, vol. 11, n° 10, p. e1005211. https://archive-ouverte.unige.ch/unige:77339

167 hits

43 downloads

Update

Deposited on : 2015-11-17

Export document
Format :
Citation style :