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unige:7705 
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Cytomegalovirus quantification in plasma by an automated real-time PCR assay |
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| Published in | Journal of Clinical Virology. 2007, vol. 38, no. 4, p. 298-303 | |
| Abstract | BACKGROUND: Sensitive quantitation of cytomegalovirus (CMV) DNA in blood is helpful for the diagnosis of CMV infection or reactivation and the monitoring of transplanted patients. OBJECTIVES: We compared a new PCR assay coupled with an automated extraction system (CMV real-time PCR, Abbott Molecular, Des Plaines, IL, USA) to a previously validated method (ultrasensitive Cobas Amplicor CMV DNA Monitor, Roche Molecular, Indianapolis, IN, USA). RESULTS: Using limiting dilutions of CMV DNA positive plasma, the two assays had a similar detection threshold ranging between 20 and 45 copies/ml. Coefficients of variation of CMV real-time PCR assay varied from 1 to 12% for CMV DNA levels between 10,000 and 20 copies/ml. Viral loads assessed by the two methods on 179 clinical samples showed an overall concordance of 89% and an excellent correlation (R=0.94). Discrepancies were only observed for samples with low CMV DNA levels (<300 copies/ml); 18 samples were positive by CMV real-time PCR only, and 2 samples by ultrasensitive Cobas CMV only. Values obtained by CMV real-time PCR were on average 0.4 log higher than those of ultrasensitive Cobas CMV. Successive samples of transplanted patients with evidence of CMV infection/reactivation revealed that CMV real-time PCR assay was positive earlier and for a longer period of time after treatment initiation. CONCLUSIONS: Although both assays had similar analytical performances, the CMV real-time PCR assay has the advantages of automated extraction and higher dynamic range, and shows a trend for an improved sensitivity that might impact on clinical decisions. | |
| Keywords | Adult — Analysis of Variance — Cytomegalovirus/ genetics — DNA, Viral/ blood — Female — Humans — Plasma/virology — Polymerase Chain Reaction/ methods — Reproducibility of Results — Sensitivity and Specificity — Viral Load/ methods | |
| Identifiers | PMID: 17321200 | |
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| Research group | Groupe Laurent Kaiser (virologie clinique) (668) | |
| Citation (ISO format) | YERLY FERRILLO, Sabine et al. Cytomegalovirus quantification in plasma by an automated real-time PCR assay. In: Journal of Clinical Virology, 2007, vol. 38, n° 4, p. 298-303. doi: 10.1016/j.jcv.2007.01.003 https://archive-ouverte.unige.ch/unige:7705 |
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