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Scientific article
English

Protective efficacy of individual CD8+ T cell specificities in chronic viral infection

Contributors

Johnson, Susan; Bergthaler, Andréas; Graw, Frederik; Flatz, Lukas Roland; Bonilla, Weldy V; Siegrist, Claire-Anne; Lambert, Paul Henri; Regoes, Roland; Pinschewer, Daniel

Published in

The Journal of immunology, vol. 194, no. 4, p. 1755-1762

Publication date

2015

Abstract

Specific CD8(+) T cells (CTLs) play an important role in resolving protracted infection with hepatitis B and C virus in humans and lymphocytic choriomeningitis virus (LCMV) in mice. The contribution of individual CTL specificities to chronic virus control, as well as epitope-specific patterns in timing and persistence of antiviral selection pressure, remain, however, incompletely defined. To monitor and characterize the antiviral efficacy of individual CTL specificities throughout the course of chronic infection, we coinoculated mice with a mixture of wild-type LCMV and genetically engineered CTL epitope-deficient mutant virus. A quantitative longitudinal assessment of viral competition revealed that mice continuously exerted CTL selection pressure on the persisting virus population. The timing of selection pressure characterized individual epitope specificities, and its magnitude varied considerably between individual mice. This longitudinal assessment of "antiviral efficacy" provides a novel parameter to characterize CTL responses in chronic viral infection. It demonstrates remarkable perseverance of all antiviral CTL specificities studied, thus raising hope for therapeutic vaccination in the treatment of persistent viral diseases.

Keywords

  • Animals
  • Epitopes, T-Lymphocyte/immunology
  • Lymphocytic Choriomeningitis/immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Cell Antigen Receptor Specificity/immunology
  • T-Lymphocytes, Cytotoxic/immunology

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

JOHNSON, Susan et al. Protective efficacy of individual CD8+ T cell specificities in chronic viral infection. In: The Journal of immunology, 2015, vol. 194, n° 4, p. 1755–1762. doi: 10.4049/jimmunol.1401771

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0022-1767
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Technical informations

Creation

16/09/2015 14:44:00

First validation

16/09/2015 14:44:00

Update

14/03/2023 23:44:05

Status update

14/03/2023 23:44:04

Last indexation

31/10/2024 01:37:45
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