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The DNA-binding domain of the Escherichia coli CpxR two-component response regulator is constitutively active and cannot be fully attenuated by fused adjacent heterologous regulatory domains

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Published in Microbiology. 2006, vol. 152, no. 2, p. 431-441
Abstract Two-component systems (TCS) based on a sensor histidine kinase and a phosphorylated cognate target regulator allow rapid responses to environmental changes. TCS are highly evolutionarily conserved, though in only a few cases are the inducing signals understood. This study focuses on the Escherichia coli CpxR response regulator that responds to periplasmic and outer-membrane stress. N-terminal deletion mutations have been isolated that render the transcription factor constitutively active, indicating that the N terminus functions, in part, to keep the C-terminal winged-helix DNA-binding effector domain in an inactive state. Analysis of truncations spanning the CpxR interdomain region revealed that mutants retaining the alpha5 helix significantly augment activation. Hybrid proteins obtained by fusing the CpxR effector domain to structurally similar heterologous N-terminal regulatory domains, or even GFP, failed to restore repression to the C-terminal domain. These findings shed light on the mechanism of CpxR effector domain activation and on the investigation of constitutive mutants obtained by truncation in other TCS.
Keywords Bacterial Proteins/chemistry/ metabolismDNA-Binding Proteins/chemistry/ physiologyEscherichia coli/ physiologyGene Expression Regulation, Bacterial/ physiologyGenes, RegulatorMutationProtein Kinases/chemistry/ physiologyProtein Structure, Tertiary
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PMID: 16436431
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Other version: http://mic.sgmjournals.org/cgi/reprint/152/2/431.pdf
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TAPPAREL, Caroline, MONOD, Antoinette, KELLEY, William. The DNA-binding domain of the Escherichia coli CpxR two-component response regulator is constitutively active and cannot be fully attenuated by fused adjacent heterologous regulatory domains. In: Microbiology, 2006, vol. 152, n° 2, p. 431-441. https://archive-ouverte.unige.ch/unige:7602

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Deposited on : 2010-06-21

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