UNIGE document Scientific Article
previous document  unige:7590  next document
add to browser collection

Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils

Stendahl, O.
Jerstrom, P.
Theler, J. M.
Clark, R. A.
Published in Science. 1994, vol. 265, no. 5177, p. 1439-1441
Abstract Subcellular gradients of cytosolic free Ca2+ concentration, [Ca2+]i, are thought to be critical for the localization of functional responses within a cell. A potential but previously unexplored mechanism for the generation of gradients of [Ca2+]i is the accumulation of Ca2+ stores at the site of Ca2+ action. The distribution of the Ca2+ store markers Ca(2+)-dependent adenosine triphosphatase and calreticulin was investigated in resting and phagocytosing human neutrophils. Both proteins showed an evenly distributed fine granular pattern in nonphagocytosing cells, but became markedly concentrated in the filamentous actin-rich cytoplasmic area around the ingested particle during phagocytosis. This redistribution began at early stages of phagocytosis and did not depend on an increase in [Ca2+]i. Thus, accumulation of Ca2+ stores in a restricted area of the cell may contribute to the generation of localized increases in [Ca2+]i.
Keywords Actins/analysisCalcium/ metabolismCalcium Channels/metabolismCalcium-Binding Proteins/metabolismCalcium-Transporting ATPases/metabolismCalreticulinFluorescent Antibody TechniqueHumansNeutrophils/immunology/ metabolism/ultrastructureOrganelles/metabolism/ultrastructurePhagocytosisPhagosomes/metabolism/ultrastructureRibonucleoproteins/metabolism
PMID: 8073285
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.jstor.org/stable/pdfplus/2884830.pdf
(ISO format)
STENDAHL, O. et al. Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils. In: Science, 1994, vol. 265, n° 5177, p. 1439-1441. https://archive-ouverte.unige.ch/unige:7590

126 hits

0 download


Deposited on : 2010-06-21

Export document
Format :
Citation style :