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Title

Turnover of CD4+ and CD8+ T lymphocytes in HIV-1 infection as measured by Ki-67 antigen

Authors
Sachsenberg, N.
Perelson, A. S.
Schockmel, G. A.
Leduc, D.
Published in Journal of Experimental Medicine. 1998, vol. 187, no. 8, p. 1295-1303
Abstract We investigated CD4+ and CD8+ T cell turnover in both healthy and HIV-1-infected adults by measuring the nuclear antigen Ki-67 specific for cell proliferation. The mean growth fraction, corresponding to the expression of Ki-67, was 1.1% for CD4(+) T cells and 1.0% in CD8(+) T cells in healthy adults, and 6.5 and 4.3% in HIV-1-infected individuals, respectively. Analysis of CD45RA+ and CD45RO+ T cell subsets revealed a selective expansion of the CD8+ CD45RO+ subset in HIV-1-positive individuals. On the basis of the growth fraction, we derived the potential doubling time and the daily turnover of CD4+ and CD8+ T cells. In HIV-1-infected individuals, the mean potential doubling time of T cells was five times shorter than that of healthy adults. The mean daily turnover of CD4+ and CD8+ T cells in HIV-1-infected individuals was increased 2- and 6-fold, respectively, with more than 40-fold interindividual variation. In patients with <200 CD4+ counts, CD4+ turnover dropped markedly, whereas CD8+ turnover remained elevated. The large variations in CD4+ T cell turnover might be relevant to individual differences in disease progression.
Keywords AdultAntigens, CD45CD4 Lymphocyte CountCD4-Positive T-Lymphocytes/ cytologyCD8-Positive T-Lymphocytes/ cytologyCell CycleFlow Cytometry/methodsHIV Infections/ immunologyHIV-1/ immunologyHalf-LifeHumansKi-67 Antigen/ analysisLymphocyte ActivationT-Lymphocyte Subsets/cytology
Identifiers
PMID: 9547340
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SACHSENBERG, N. et al. Turnover of CD4+ and CD8+ T lymphocytes in HIV-1 infection as measured by Ki-67 antigen. In: Journal of Experimental Medicine, 1998, vol. 187, n° 8, p. 1295-1303. https://archive-ouverte.unige.ch/unige:7560

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Deposited on : 2010-06-21

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